LOCUS       sequence-138997-        1377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of
            Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash1
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             212..229
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 gagagtgctg aagaacgagc agggggatct ccggcgagct ctcgagaatt ctcacgcgtc
       61 tgcaggatat caagcttgcg ttaccggatc catgggagag aaaccaggga ccagggtctt
      121 caagaagtcg agccctaact gcaagctcac cgtgtacttg ggcaagcgtg actttgtgga
      181 tcacttggac aaagtggatc ctgtcgatgg ttgttgtcct ggttgttgtg tggtgcttgt
      241 ggatcctgac tacttgaagg accggaaagt gtttgtgacc ctcacctgtg ccttccgcta
      301 tggccgagaa gacctggatg tactgggcct gtctttccgc aaagatctgt tcatcgccac
      361 ctaccaggcc ttccccccca tgcccaaccc acctcggccc cccacccgcc tacaggaccg
      421 actgctgaag aagttgggcc agcatgccca cccctttttt ttcacaatac cccagaattt
      481 gccttgctcc gtcacactgc agccaggacc ggaggacaca gggaaggcct gtggagtaga
      541 ctttgagatt cgagccttct gtgccaaatc tatagaagaa aaaagccaca aaaggaactc
      601 cgtgcggctt atcatcagaa aggtacagtt tgctcctgag acacccggcc cccagccatc
      661 agctgaaacc acacgccact tcctcatgtc tgaccggagg tccctgcacc tagaggcttc
      721 cctggacaaa gagctgtact accatgggga acccctcaat gtcaacgtcc acgtcaccaa
      781 caattctgcc aagaccgtca agaagatcag agtgtctgtg agacagtatg ccgacatttg
      841 cctcttcagc accgcgcagt acaagtgtcc tgtggctcag cttgaacaag atgaccaggt
      901 gtctcccagt tccacattct gcaaggtgta caccataacc ccgctgctca gtgacaaccg
      961 agagaagcgt ggccttgccc ttgatgggca actcaagcac gaagacacca acctggcttc
     1021 cagcaccatt gtgaaggagg gagccaacaa ggaggtgctg ggaatcctag tatcctacag
     1081 ggtcaaggtg aagctggtgg tgtctcgagg cggggatgtc tccgtggagc tacctttcgt
     1141 cctaatgcac cccaagcccc acgaccacat cacccttccc cgaccccagt cagccccccg
     1201 ggaaatagac atccctgtgg ataccaacct cattgaattc gataccaact atgccacaga
     1261 cgacgacatc gtgtttgagg actttgcgag gcttcggctg aaggggatga aggatgacga
     1321 ctgtgatgac cagttctgct agtctagagg gcccgggatc caccggatct agataac
//
