LOCUS       sequence-139001-        1377 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash4
FEATURES             Location/Qualifiers
     source          1..1377
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             767..784
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 gagagtgctg aagaacgagc agggggatct ccggcgagct ctcgagaatt ctcacgcgtc
       61 tgcaggatat caagcttgcg ttaccggatc catgggtgaa aaaccaggga ccagggtctt
      121 caagaagtcg agccctaact gcaagctcac cgtgtacttg ggcaagcgtg actttgtgga
      181 tcacttggac aaagtggatc ctgtcgatgg tgtggtgctt gtggatcctg actacttgaa
      241 ggaccggaaa gtgtttgtga ccctcacctg tgccttccgc tatggccgag aagacctgga
      301 tgtactgggc ctgtctttcc gcaaagatct gttcatcgcc acctaccagg ccttcccccc
      361 catgcccaac ccacctcggc cccccacccg cctacaggac cgactgctga agaagttggg
      421 ccagcatgcc cacccctttt ttttcacaat accccagaat ttgccttgct ccgtcacact
      481 gcagccagga ccggaggaca cagggaaggc ctgtggagta gactttgaga ttcgagcctt
      541 ctgtgccaaa tctatagaag aaaaaagcca caaaaggaac tccgtgcggc ttatcatcag
      601 aaaggtacag tttgctcctg agacacccgg cccccagcca tcagctgaaa ccacacgcca
      661 cttcctcatg tctgaccgga ggtccctgca cctagaggct tccctggaca aagagctgta
      721 ctaccatggg gaacccctca atgtcaacgt ccacgtcacc aacaattgtt gtcctggttg
      781 ttgttctgcc aagaccgtca agaagatcag agtgtctgtg agacagtatg ccgacatttg
      841 cctcttcagc accgcgcagt acaagtgtcc tgtggctcag cttgaacaag atgaccaggt
      901 gtctcccagt tccacattct gcaaggtgta caccataacc ccgctgctca gtgacaaccg
      961 agagaagcgt ggccttgccc ttgatgggca actcaagcac gaagacacca acctggcttc
     1021 cagcaccatt gtgaaggagg gagccaacaa ggaggtgctg ggaatcctag tatcctacag
     1081 ggtcaaggtg aagctggtgg tgtctcgagg cggggatgtc tccgtggagc tacctttcgt
     1141 cctaatgcac cccaagcccc acgaccacat cacccttccc cgaccccagt cagccccccg
     1201 ggaaatagac atccctgtgg ataccaacct cattgaattc gataccaact atgccacaga
     1261 cgacgacatc gtgtttgagg actttgcgag gcttcggctg aaggggatga aggatgacga
     1321 ctgtgatgac cagttctgct agtctagagg gcccgggatc caccggatct agataac
//
