LOCUS       sequence-139004-        1420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash6
FEATURES             Location/Qualifiers
     source          1..1420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1322..1339
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 gagagtgctg aagaacgagc agggggatct ccggcgagct ctcgagaatt ctcacgcgtc
       61 tgcaggatat caagcttgcg ttaccggatc catgggagag aaaccaggga ccagggtctt
      121 caagaagtcg agccctaact gcaagctcac cgtgtacttg ggcaagcgtg actttgtgga
      181 tcacttggac aaagtggatc ctgtcgatgg tgtggtgctt gtggatcctg actacttgaa
      241 ggaccggaaa gtgtttgtga ccctcacctg tgccttccgc tatggccgag aagacctgga
      301 tgtactgggc ctgtctttcc gcaaagatct gttcatcgcc acctaccagg ccttcccccc
      361 catgcccaac ccacctcggc cccccacccg cctacaggac cgactgctga agaagttggg
      421 ccagcatgcc cacccctttt ttttcacaat accccagaat ttgccttgct ccgtcacact
      481 gcagccagga ccggaggaca cagggaaggc ctgtggagta gactttgaga ttcgagcctt
      541 ctgtgccaaa tctatagaag aaaaaagcca caaaaggaac tccgtgcggc ttatcatcag
      601 aaaggtacag tttgctcctg agacacccgg cccccagcca tcagctgaaa ccacacgcca
      661 cttcctcatg tctgaccgga ggtccctgca cctagaggct tccctggaca aagagctgta
      721 ctaccatggg gaacccctca atgtcaacgt ccacgtcacc aacaattctg ccaagaccgt
      781 caagaagatc agagtgtctg tgagacagta tgccgacatt tgcctcttca gcaccgcgca
      841 gtacaagtgt cctgtggctc agcttgaaca agatgaccag gtgtctccca gttccacatt
      901 ctgcaaggtg tacaccataa ccccgctgct cagtgacaac cgagagaagc gtggccttgc
      961 ccttgatggg caactcaagc acgaagacac caacctggct tccagcacca ttgtgaagga
     1021 gggagccaac aaggaggtgc tgggaatcct agtatcctac agggtcaagg tgaagctggt
     1081 ggtgtctcga ggcggggatg tctccgtgga gctacctttc gtcctaatgc accccaagcc
     1141 ccacgaccac atcacccttc cccgacccca gtcagccccc cgggaaatag acatccctgt
     1201 ggataccaac ctcattgaat tcgataccaa ctatgccaca gacgacgaca tcgtgtttga
     1261 ggactttgcg aggcttcggc tgaaggggat gaaggatgac gactgtgatg accagttctg
     1321 ctgttgtcct ggttgttgtt agtctagagg gcccgggatc caccggatct agataactga
     1381 tcataatcag ccataccaca tttgtagagg ttttacttgc
//
