LOCUS       sequence-139007-        7958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3F protein (truncated at cleavage site P3; ie, 
            long and contains a 93 nt deletion in exon 3), C-terminally fused to
            the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3f(L,-)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4538..4555
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4581..4702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4618..4637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4672..4691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4740..4757)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4911..5499)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4991..5010)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5670..6461,6462..6530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6293..6312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6531..6635)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6703..6721
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6759..6781)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6783..6803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6788..7118
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6969..7104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7031..7050
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7255..7274
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7341..7796)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7478..7499)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7690..7709
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7922..7944
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7936..7953
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7937..7953
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcttgtcac
     1621 tgccttcatc ctctgggctt ccctgctgac cggggcctgg cctgctaccc ctatccagga
     1681 ccagcttcca gccacaccta gagtccgact gtcattcaaa gaacttaagg ccaccggtac
     1741 cgcccacttc ttcaactttc tgctcaacac tacagactac agaatcctgc tcaaggatga
     1801 ggaccatgac cgcatgtatg tgggcagcaa ggactacgtg ctgtccctgg acctgcatga
     1861 catcaaccga gagcccctta ttatccattg ggcagcctcc ccgcagcgca ttgaggagtg
     1921 catactgtca ggcaaggatg gcaatggaga gtgtggtaac ttcgtccggc tcatccagcc
     1981 ttggaaccga acacacctgt atgtgtgtgg gaccggtgcc tacaacccca tgtgcaccta
     2041 tgtgaaccgt ggccgtcgcg cacaggatta catcttctac ctggagcctg agaaactgga
     2101 gtcaggaaaa ggcaaatgtc cctatgaccc caagctggac acagcctcag ccctcatcaa
     2161 tgaggaactc tatgcaggag tgtacatcga ttttatgggt accgatgcag ccattttccg
     2221 cacgctgggg aagcagacag ccatgcgcac agatcagtac aactcccggt ggctcaatga
     2281 tccttccttc atacatgctg agctcatccc tgacagcgca gagcgcaatg acgataaact
     2341 ctacttcttc ttccgagagc gctcggcaga ggcgccacag aaccctgctg tgtatgcccg
     2401 catcgggcgc atctgcctca acgatgacgg tggccattgc tgcctggtca acaagtggag
     2461 cacattcctg aaggctcggc tcgtctgctc cgtgccaggc gaggatggta tcgagaccca
     2521 cttcgacgaa ctccaggatg tgtttgtcca gcagacccag gacatcagga acccagtcat
     2581 ttatgctgtc tttacctctt caggctctgt gttccgaggg tctgcagtgt gtgtctactc
     2641 catggctgat atccgcatgg tcttcaatgg gccttttgct cacaaggagg gccccaacta
     2701 tcaatggatg cccttctcag gaaagatgcc ctatcctcgg cctggcacat gccctggtgg
     2761 aaccttcacg ccctcgatga agtccactaa ggactaccct gatgaagtga tcaacttcat
     2821 gcgcactcat ccgctcatgt accaggccgt ctacccgctc cagcggcgac ccctggtggt
     2881 ccgtacaggc gctccctacc gcctcaccac tgtcgctgtg gaccaggtgg atgcagctga
     2941 tggacgctat gaggtgcttt tcctgggcac agaccgcggg acagtgcaga aggtcatcgt
     3001 gctgcccaag gacgaccagg aggtggaaga gctgatgcta gaggaagtgg aggtcttcaa
     3061 ggaaccagca cctgttaaga ccatgaccat ctcttccaag aggcaacaac tgtatgtggc
     3121 ctcagccgtg ggtgtcacac acctgagcct gcaccgctgc caggcctatg gagctgcctg
     3181 tgccgactgc tgccttgccc gcgatcccta ctgtgcctgg gatggccagg cctgctctcg
     3241 ctacacagca tcctccaaga ggcggagtcg ccggcaagat gtccgccacg ggaaccctat
     3301 cagacagtgc cgtggtttca attccaatgc aaacaagaat gccgtagagt ctgtgcagta
     3361 cggagtggcc ggcagcgcag ctttccttga gtgtcagccc cgctcacctc aagccactgt
     3421 caagtggctg ttccagcgag atcccagtga tcggcgccgt gagattcgcg cagaggaccg
     3481 cttcctgcgt acagagcagg ggctgttgct tcgcgccctg cagcttggtg accgcggcct
     3541 ctactcctgc acggccactg agaacaactt caagcacatc gtcacgcggg tgcagctgca
     3601 tgtactgggc cgggatgccg tccatgctgc cctcttcccc ccactggctg tgagcgtccc
     3661 tccacctcct ggcacaggcc ctccaacgcc tccttaccag gagctggccc agctcctagc
     3721 ccagccggaa gtgggcctca tccatcagta ctgccagggt tactggcgcc acgtgccccc
     3781 cagacccagg gaggcgccag gagcacttag gcctcctgaa ctccaggacc agaaaaagcc
     3841 gggcgcgcct gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg
     3901 accgtcagtc ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc
     3961 tgaggtcaca tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg
     4021 gtacgtggac ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa
     4081 cagcacgtac cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa
     4141 ggagtacaag tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc
     4201 caaagccaaa gggcagcccc gagaaccaca ggtgtatacc ctgcccccat cccgggatga
     4261 gctgaccaag aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat
     4321 cgccgtggag tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt
     4381 gctggactcc gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg
     4441 gcagcagggg aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac
     4501 gcagaagagc ctctccctgt ctccgggtaa aggcggccat catcaccatc accattaatc
     4561 tagagatctt attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca
     4621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4681 ccaaactcat caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc
     4741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5101 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5341 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5641 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5701 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5761 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5821 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5881 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5941 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6001 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6061 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6121 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6181 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6241 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6301 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6361 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6421 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6481 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6541 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6601 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6661 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6721 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     6781 gaggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta
     6841 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6961 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7021 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7081 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7141 ccattttcgt cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     7201 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     7261 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     7321 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     7381 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     7441 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7501 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7561 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7621 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7681 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7741 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     7801 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7861 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7921 tcccagtcac gacgttgtaa aacgacggcc agtgaatt
//
