LOCUS       sequence-139018-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-copGFP
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..700
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(838..858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 aattggatcc accatggaga gcgacgagag cggcctgccc gccatggaga tcgagtgccg
       61 catcaccggc accctgaacg gcgtggagtt cgagctggtg ggcggcggag agggcacccc
      121 caagcagggc cgcatgacca acaagatgaa gagcaccaaa ggcgccctga ccttcagccc
      181 ctacctgctg agccacgtga tgggctacgg cttctaccac ttcggcacct accccagcgg
      241 ctacgagaac cccttcctgc acgccatcaa caacggcggc tacaccaaca cccgcatcga
      301 gaagtacgag gacggcggcg tgctgcacgt gagcttcagc taccgctacg aggccggccg
      361 cgtgatcggc gacttcaagg tggtgggcac cggcttcccc gaggacagcg tgatcttcac
      421 cgacaagatc atccgcagca acgccaccgt ggagcacctg caccccatgg gcgataacgt
      481 gctggtgggc agcttcgccc gcaccttcag cctgcgcgac ggcggctact acagcttcgt
      541 ggtggacagc cacatgcact tcaagagcgc catccacccc agcatcctgc agaacggggg
      601 ccccatgttc gccttccgcc gcgtggagga gctgcacagc aacaccgagc tgggcatcgt
      661 ggagtaccag cacgccttca agacccccat tgccttcgcc agatcccgcg ctcagtcgtc
      721 caattctgcc gtggacggca ccgccggacc cggctccacc ggatctcgct aagaattcgt
      781 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
      841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
      901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
      961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     1021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggacttt
//
