LOCUS       sequence-139019-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDH-EF1-copGFP
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..769)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     intron          818..1021
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(841..861)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttgtcgacga attcttagcg agatccggtg gagccgggtc
       61 cggcggtgcc gtccacggca gaattggacg actgagcgcg ggatctggcg aaggcaatgg
      121 gggtcttgaa ggcgtgctgg tactccacga tgcccagctc ggtgttgctg tgcagctcct
      181 ccacgcggcg gaaggcgaac atggggcccc cgttctgcag gatgctgggg tggatggcgc
      241 tcttgaagtg catgtggctg tccaccacga agctgtagta gccgccgtcg cgcaggctga
      301 aggtgcgggc gaagctgccc accagcacgt tatcgcccat ggggtgcagg tgctccacgg
      361 tggcgttgct gcggatgatc ttgtcggtga agatcacgct gtcctcgggg aagccggtgc
      421 ccaccacctt gaagtcgccg atcacgcggc cggcctcgta gcggtagctg aagctcacgt
      481 gcagcacgcc gccgtcctcg tacttctcga tgcgggtgtt ggtgtagccg ccgttgttga
      541 tggcgtgcag gaaggggttc tcgtagccgc tggggtaggt gccgaagtgg tagaagccgt
      601 agcccatcac gtggctcagc aggtaggggc tgaaggtcag ggcgcctttg gtgctcttca
      661 tcttgttggt catgcggccc tgcttggggg tgccctctcc gccgcccacc agctcgaact
      721 ccacgccgtt cagggtgccg gtgatgcggc actcgatctc catggcgggc aggccgctct
      781 cgtcgctctc catggtggat ccaattcctc acgacacctg aaatggaaga aaaaaacttt
      841 gaaccactgt ctgaggcttg agaatgaacc aagatccaaa ctcaaaaagg gcaaattcca
      901 aggagaatta catcaagtgc caagctggcc taacttcagt ctccacccac tcagtgtggg
      961 gaaactccat cgcataaaac ccctcccccc aacctaaaga cgacgtactc caaaagctcg
     1021 agaactaatc
//
