LOCUS       sequence-139031-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding centrin-1 with an N-terminal FLAG and 
            dTomato tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-FLAG-dTomato-centrin-1
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             81..779
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(211..229)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     479..498
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     673..696
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtatggatt
       61 acaaggatga cgacgataag gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
      121 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
      181 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
      241 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
      301 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
      361 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
      421 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
      481 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
      541 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
      601 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
      661 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      721 acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt
      781 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc atggcttccg
      841 gcttcaagaa gcccagcgct gcctccaccg gccaaaagag aaaggtggca cctaagcccg
      901 agctcactga ggatcagaag caagaagttc gggaagcatt tgacctcttc gacgtggacg
      961 gaagtgggac catcgac
//
