LOCUS       sequence-139036-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-EGFP-C1-centrin-1
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(129..131,132..134,135..845)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(129..131,132..134,135..845)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(174..195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(435..454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     782..803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgactcta ctagaggatc gctagcgcta ccggactcag
      121 atctcgacat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      181 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      241 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      301 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      361 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      421 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      481 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      541 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      601 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      661 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      721 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      781 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      841 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccatgg
      901 cttccggctt caagaagccc agcgctgcct ccaccggcca aaagagaaag gtggcaccta
      961 agcccgagct cactgaggat cagaagcaag aagttcggga agcatttgac ctcttcgacg
     1021 tggacggaag tggg
//
