LOCUS       sequence-139037-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             620..642
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    657..681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(681..700)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(682..700)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agtatcattt ggcccacgga aagtgaccgc ggtgatttct gaacaaacaa caaggactcg
       61 agcgcccttg ttgttttcag ccaagtcctt ggctaaccga agaaccgtgc caccagcaaa
      121 gcaaccttgt tggtacatca taagcctctt gaccgatgga cgaagcccaa gtagcttagt
      181 gagttgatag tcacacccag gcatgtccac accactggtt gtgcaaaaga ccaaatgggt
      241 aattttggac ttgggctggc cccattcctt gatggccttt tgagctgcct ctttgccaag
      301 tttgggcact tcaaccacca ctatgtcttg cctagcatca agagaaggtg ccatgtattc
      361 acacatacta ggattctctt tcaagatttc ctctgttaag tgcatgtacc ttttcttaat
      421 cattgatttt tcacacatgc gcttaaattt ctccttaaga tcagtcttgt gctcactgtt
      481 agtgatacga aaataaaaat cagggtaagt gctttgatca acacagtttg aaggtgtggc
      541 tgttccaatg gccatgacag tggctggacc ttcagcacgt tgtgccttac gatactcctc
      601 gactgtcacc atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa
      661 ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcgg
//
