LOCUS       sequence-139040-       11288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding cenexin with an N-terminal EGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11288)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-EGFP-C3-cenexin
FEATURES             Location/Qualifiers
     source          1..11288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3304..3803
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3339..3362)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3736..3755
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3824..4423
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3824..3843)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4320..4340
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4437..5025
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4490..4510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4908..4919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5232..5865
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5993..6009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5993..6009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6006..6028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6017..6033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6041..6047,6048..6065,6066..6071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6086..6107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6224..6241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6395..6983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6475..6494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7154..7945,7946..8014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7777..7796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8015..8119)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8167..8301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8204..8223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8258..8277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8469..9102
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9149..9274
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9771..10004
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10189..10233
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10496..10611
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10669..10972
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10973..11176
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11127..11147
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11173..11197
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtac
      721 tcagatctcg acatgaagga ccgctcttca actcccccct tacatgttca cgtggatgag
      781 aacacccctg tccacgtcca cataaaaaaa ctcccgaaac catcagcgac cagcagccag
      841 aaatctcaca agcgaggaat gaaaggggac actgtgaatg tgcggcggag tgtccgggtg
      901 aaaaccaagg taccttggat gccccctgga aaatcatctg cccggcctgt gggatgcaag
      961 tgggagaatc cacctcattg cctggagatc acgccaccat cttcagaaaa gctggtctca
     1021 gtgatgcggt taagtgacct ctctacagaa gatgatgact caggtcactg taaaatgaac
     1081 cgttatgata agaagattga tagtctaatg aatgcggttg gttgtctgaa gtctgaggtc
     1141 aagatgcaaa aaggtgagcg ccagatggcc aaaaggttcc tggaggaacg gaaggaagag
     1201 ctggaggagg tggcccacga actggctgag actgagcacg agaacacggt gttgaggcac
     1261 aacatcgagc gcatgaagga ggagaaggac ttcaccatac ttcagaagaa acacctacaa
     1321 caggagaagg agtgcctcat gtccaagctg gtggaggcgg aaatggatgg ggctgcggct
     1381 gccaagcagg tcatggcctt gaaggatacc atcgggaagc tgaaaacgga gaaacaaatg
     1441 acctgcacgg acatcaacac cctgacaagg cagaaggaac ttctcctgca gaagctgagc
     1501 acatttgagg agaccaaccg caccctccga gacctcctga gggaacagca ctgcaaagag
     1561 gattctgaaa gactaatgga gcaacaagga gcactgctga aacggctggc ggaggccgac
     1621 tcagagaaag cgcgcctgct gttactgctg caagacaagg acaaggaggt ggaagagctc
     1681 cttcaggaaa tacaatgtga gaaggctcaa gcaaagacag cctctgagct ttctaaatcc
     1741 atggagtcca tgcgtgggca tttgcaggca cagcttcggt ccaaagaggc tgagaacagt
     1801 cgcctgtgca tgcagattaa gaatctggag cgcagcggga atcagcataa ggcagaagtg
     1861 gaggccatca tggagcagct gaaggagttg aagcagaagg gagaccgaga caaagagagc
     1921 ttgaagaagg ccatccgagc ccagaaggag cgagccgaga agagcgagga gtatgctgag
     1981 cagctacacg tgcaactcgc tgacaaggat ctttatgtcg ctgaagcttt atccactctg
     2041 gaatcctgga ggagccgcta caaccaagtt gtaaaagaaa agggagacct tgagctggaa
     2101 attattgtcc tgaatgaccg ggtaacagat cttgtaaacc aacaacaaac cctggaggag
     2161 aagatgcggg aagaccggga tagcctggtg gagagactac accgtcagac tgctgagtat
     2221 tccgcattca agctggagaa tgagaggctg aaggccagct ttgctccaat ggaggacaaa
     2281 ctcaaccagg cacacctcga ggtccagcag ctgaaggcct cagtgaagaa ctatgagggg
     2341 atgattgaca actataagag tcaggtgatg aagaccagat tggaggctga tgaagtagct
     2401 gcccagctag aacgctgtga caaagagaac aagatcctta aagatgagat gaacaaagag
     2461 attgaggcgg cacgaaggca gttccagtct cagctggctg acctgcagca gctccctgac
     2521 atcctgaaga tcacggaggc gaagctggct gagtgccaag accaactgca gggctatgag
     2581 cggaagaaca tcgacctcac agccatcata tcagacctgc gcagccggat cgaacaccag
     2641 ggggacaagc tggagatggc gagagagaaa catcaggctt cccagaagga aaataaacag
     2701 ctgagtctga aggtggatga actggagagg aaactggagg cgaccagtgc ccagaatatc
     2761 gagttcctac aggtgattgc caagagggag gaggcaatcc accagtccca gctgcggctg
     2821 gaggagaaaa cacgggaatg tgggaccctg gcaaggcagt tggagagtgc cattgaagat
     2881 gcgaggaggc aggtggaaca aaccaaggag cacgcactct ccaaggagcg agcagcccag
     2941 aacaaaatcc tggaccttga gacccagctg agcagaacca aaacggaatt gagccagctg
     3001 cggcggagcc gtgatgatgc ggaccgccgc taccagagcc ggctgcaaga cctgaaagat
     3061 cgcctggagc agtccgagag caccaaccgc agcatgcaga actacgtcca gttcctcaaa
     3121 tcatcatacg ccaacgtgtt tggggatggt ccctattcca ccttcctgac tagctctccc
     3181 atccgctccc gatctcctcc tgcctgagga tccgcggccg cgaccctagg ccgacgcgtc
     3241 cggttaaccg ggatcgagat cctgcagaac cacgagtctg gttgcattct agataattct
     3301 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     3361 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     3421 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     3481 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     3541 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     3601 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     3661 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     3721 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     3781 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     3841 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     3901 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     3961 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     4021 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     4081 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     4141 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     4201 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     4261 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     4321 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     4381 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc
     4441 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4501 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4561 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4621 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4681 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4741 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4801 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     4861 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     4921 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     4981 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     5041 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     5101 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     5161 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5221 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     5281 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     5341 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     5401 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     5461 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     5521 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     5581 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     5641 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     5701 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5761 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5821 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     5881 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     5941 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     6001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8161 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8281 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     8341 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     8401 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     8461 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     8521 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     8581 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     8641 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     8701 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     8761 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     8821 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     8881 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     8941 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9001 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9061 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     9121 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     9181 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     9241 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     9301 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     9361 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     9421 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     9481 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     9541 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     9601 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     9661 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     9721 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     9781 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     9841 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     9901 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     9961 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
    10021 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
    10081 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
    10141 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
    10201 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
    10261 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
    10321 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
    10381 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
    10441 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa
    10501 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
    10561 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
    10621 ttacagggac agcagagatc cagtttatcg ataagcttgg gagttccgcg ttacataact
    10681 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    10741 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    10801 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    10861 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10921 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    10981 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    11041 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    11101 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    11161 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
    11221 ttttgacctc catagaagac accgactcta ctagaggatc gctagcgcta ccggactcag
    11281 atctcgac
//
