LOCUS       sequence-139043-        9302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding centrin-1 with an N-terminal FLAG and 
            dTomato tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9302)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-FLAG-dTomato-centrin-1
FEATURES             Location/Qualifiers
     source          1..9302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             28..726
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(158..176)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     426..445
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     620..643
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        1393..1892
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1428..1451)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1825..1844
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1913..2512
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1913..1932)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2409..2429
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2526..3114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2579..2599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2997..3008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3321..3954
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4082..4098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4082..4098)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4095..4117)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4106..4122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4130..4136,4137..4154,4155..4160))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4175..4196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4313..4330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4484..5072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4564..4583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5243..6034,6035..6103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5866..5885
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6104..6208)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6256..6390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6293..6312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6347..6366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6558..7191
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7238..7363
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7860..8093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8278..8322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8585..8700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        8758..9061
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9062..9265
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9216..9236
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggattaca aggatgacga cgataaggtg agcaagggcg aggaggtcat caaagagttc
       61 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
      121 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      181 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
      241 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
      301 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
      361 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
      421 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
      481 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
      541 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
      601 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
      661 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
      721 tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccatg
      781 gcttccggct tcaagaagcc cagcgctgcc tccaccggcc aaaagagaaa ggtggcacct
      841 aagcccgagc tcactgagga tcagaagcaa gaagttcggg aagcatttga cctcttcgac
      901 gtggacggaa gtgggaccat cgacgcgaag gagctgaagg tggccatgag agcgctgggc
      961 ttcgaaccca ggaaggaaga gatgaagaaa atgatctccg aggtggacag ggaaggcacg
     1021 gggaagatca gcttcaatga cttcctggcc gtgatgacgc agaagatgtc cgagaaggac
     1081 accaaagaag aaatcctgaa ggccttcagg ctctttgatg acgatgagac cgggaagatc
     1141 tcgttcaaaa acctgaagcg tgtggccaac gagctggggg agaacctcac ggatgaggag
     1201 ctgcaggaga tgatcgacga agctgatcgg gatggggacg gcgaagtgaa cgaggaggag
     1261 ttccttcgga tcatgaagaa gaccagcctt tactgaggat ccgcggccgc gaccctaggc
     1321 cgacgcgtcc ggttaaccgg gatcgagatc ctgcagaacc acgagtctgg ttgcattcta
     1381 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     1441 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     1501 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     1561 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     1621 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     1681 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     1741 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     1801 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     1861 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     1921 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     1981 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     2041 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     2101 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     2161 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     2221 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     2281 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     2341 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     2401 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     2461 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
     2521 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2581 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2641 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2701 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2761 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2821 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2881 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     2941 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3001 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3061 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     3121 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     3181 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     3241 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3301 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     3361 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     3421 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     3481 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     3541 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     3601 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     3661 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     3721 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     3781 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3841 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3901 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     3961 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4021 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     4081 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4141 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4201 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4261 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4321 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4381 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4441 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4501 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4561 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4621 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4681 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4741 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4801 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4861 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4921 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4981 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5041 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5101 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5161 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5221 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5281 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5341 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5401 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5461 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5521 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5581 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5641 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5701 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5761 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5821 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5881 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5941 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6001 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6061 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6241 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6301 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6361 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
     6421 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
     6481 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     6541 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     6601 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     6661 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     6721 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     6781 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     6841 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     6901 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     6961 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     7021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7201 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7261 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7321 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7381 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7441 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7501 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7561 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7621 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7681 taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg
     7741 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     7801 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7861 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     7921 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7981 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8041 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8101 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8161 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     8221 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     8281 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     8341 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     8401 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     8461 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     8521 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     8581 cgcctttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8641 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8701 tcgggtttat tacagggaca gcagagatcc agtttatcga taagcttggg agttccgcgt
     8761 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     8821 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     8881 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     8941 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     9001 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     9061 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     9121 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     9181 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     9241 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
     9301 gt
//
