LOCUS       sequence-139044-        9368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding centrin-1 with an N-terminal EGFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9368)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-EGFP-C1-centrin-1
FEATURES             Location/Qualifiers
     source          1..9368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1384..1883
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1419..1442)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1816..1835
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1904..2503
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1904..1923)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2400..2420
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2517..3105
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2570..2590)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2988..2999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3312..3945
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4073..4089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4073..4089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4086..4108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4097..4113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4121..4127,4128..4145,4146..4151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4166..4187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4304..4321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4475..5063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4555..4574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5234..6025,6026..6094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5857..5876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6095..6199)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6247..6381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6284..6303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6338..6357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6549..7182
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7229..7354
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7851..8084
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8269..8313
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8576..8691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        8749..9052
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9053..9256
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9207..9227
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9253..9277
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccat ggcttccggc
      781 ttcaagaagc ccagcgctgc ctccaccggc caaaagagaa aggtggcacc taagcccgag
      841 ctcactgagg atcagaagca agaagttcgg gaagcatttg acctcttcga cgtggacgga
      901 agtgggacca tcgacgcgaa ggagctgaag gtggccatga gagcgctggg cttcgaaccc
      961 aggaaggaag agatgaagaa aatgatctcc gaggtggaca gggaaggcac ggggaagatc
     1021 agcttcaatg acttcctggc cgtgatgacg cagaagatgt ccgagaagga caccaaagaa
     1081 gaaatcctga aggccttcag gctctttgat gacgatgaga ccgggaagat ctcgttcaaa
     1141 aacctgaagc gtgtggccaa cgagctgggg gagaacctca cggatgagga gctgcaggag
     1201 atgatcgacg aagctgatcg ggatggggac ggcgaagtga acgaggagga gttccttcgg
     1261 atcatgaaga agaccagcct ttactgagga tccgcggccg cgaccctagg ccgacgcgtc
     1321 cggttaaccg ggatcgagat cctgcagaac cacgagtctg gttgcattct agataattct
     1381 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     1441 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     1501 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     1561 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     1621 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     1681 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     1741 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     1801 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     1861 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     1921 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     1981 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     2041 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     2101 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     2161 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     2221 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     2281 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     2341 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     2401 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     2461 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaccgcgtc tggaacaatc
     2521 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2581 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2641 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2701 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2761 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2821 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2881 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     2941 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3001 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3061 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     3121 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     3181 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     3241 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3301 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     3361 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     3421 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     3481 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     3541 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     3601 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     3661 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     3721 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     3781 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3841 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3901 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     3961 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     4021 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     4081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     6241 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6361 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     6421 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     6481 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     6541 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     6601 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     6661 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     6721 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     6781 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     6841 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     6901 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     6961 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     7021 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7081 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7141 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     7201 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     7261 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     7321 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     7381 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     7441 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     7501 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     7561 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     7621 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     7681 cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     7741 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     7801 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     7861 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     7921 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     7981 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8041 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8101 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8161 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8221 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8281 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8341 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8401 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8461 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     8521 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa
     8581 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     8641 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     8701 ttacagggac agcagagatc cagtttatcg ataagcttgg gagttccgcg ttacataact
     8761 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     8821 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     8881 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     8941 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     9001 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     9061 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9121 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9181 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9241 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     9301 ttttgacctc catagaagac accgactcta ctagaggatc gctagcgcta ccggactcag
     9361 atctcgac
//
