LOCUS       sequence-139047-       11165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11165)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-EGFP-C3-Cep123
FEATURES             Location/Qualifiers
     source          1..11165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3181..3680
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3216..3239)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3613..3632
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3701..4300
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3701..3720)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4197..4217
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4314..4902
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4367..4387)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4785..4796)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5109..5742
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5870..5886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5870..5886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5883..5905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5894..5910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5918..5924,5925..5942,5943..5948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5963..5984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6101..6118)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6272..6860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6352..6371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7031..7822,7823..7891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7654..7673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7892..7996)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8044..8178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8081..8100)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8135..8154
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8346..8979
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9026..9151
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9648..9881
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10066..10110
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10373..10488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10546..10849
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10850..11053
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11004..11024
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11050..11074
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtac
      721 tcagatctcg acatgctcct gggatttcgg agaggccgca ggagtcattt caaacacatc
      781 atccatggcc ttttacctgc agccagcgtt gctccgaagg cagctgtgcc acgcacacct
      841 cctccccgca gccccaaccc atctccagag agaccaagat ctgctctggc agcagccatt
      901 ctggcgacaa cattgactgg gcggacggtt gctattcctc agcctcgcca gaggtcccgg
      961 tctgagagtg atgtgagcag tgttgaacag gacagcttca tcgagcccta tgccaccacc
     1021 tcacagctga ggcctcgacc aaattggcag agtgagatgg gaagaagatc ttctttgcca
     1081 tcctttgaaa cactggacta tggggacgaa gaggacattg aaactcagct gtcatccagc
     1141 ggcaaggaat tgggggatgt cagtgcccgg gaggacagag gaggccacag tgatgacctg
     1201 tacgctgtgc cacacagaaa tcaggtgcca ttgttacatg aggtgaacag tgaagacgat
     1261 gaaaatattt ctcatcaaga tgggtttcca ggctcccctc ctgcaccaca gcggacacaa
     1321 caaaaagatg gtaaacaccc tgttctgaat ttaaaggatg aaaaacctcc attatgtgag
     1381 aaacctccac cctccccaga tataactggt agagcacgtc aaagatatac agaaataacc
     1441 agagaaaagt ttgaggcatt aaaagaagaa aatatggacc taaacaatat gaatcaaagc
     1501 cttacccttg aactaaacac aatgaaacaa gcaatgaaag aactacagtt aaaacttaag
     1561 ggaatggaaa aagagaagag aaagctcaaa gaggctgaga aggcgtcgtc acaggaagtt
     1621 gctgcacctg aattacttta tctgcgaaaa caagctcaag aactggtgga tgaaaatgat
     1681 ggattgaaaa tgactgtcca tcgtttgaat gtagaactca gtcgatatca gacaaaattc
     1741 aggcatttgt ccaaggaaga gagcttaaat attgaaggcc tcccatccaa gggccctata
     1801 ccaccctggt tgttggatat aaagtacctg tcaccattgt tgctggctta tgaagatatg
     1861 atgaaagaga aggacgagct caatgccacc ctcaaggagg aaatgagaat gtttaggatg
     1921 cgagtccaag aagtggtgaa agaaaatgaa gaattgcacc aagagttaaa taagagtagt
     1981 gctgttacca gtgaggaatg gcgtcagctt cagactcaag caaaactggt tttagaggaa
     2041 aacaagttgt tgctggagca gttggagatt cagcaaagga aagccaagga cagccaccag
     2101 gagcgcctcc aagaagtttc taagctgact aaacaactaa tgctcctgga ggcaaaaacc
     2161 cacggccagg aaaaggagct ggcggagaac agggaacagc tggagatttt acgtgccaaa
     2221 tgccaagaac tcaaaacaca ctcggatggc aaaatcgcag tggaagttca taaatcaatt
     2281 gtgaatgaat taaaaagcca attacagaag gaagaagaga aagaaagggc tgagatggag
     2341 gagttgatgg agaagctgac agtcctgcaa gcgcagaaga agagcctgct gttagagaag
     2401 aacagtttga cagagcaaaa caaagcactg gaagccgaac ttgaacgagc acagaaaatc
     2461 aataggaaat ctcaaaagaa aattgaggtc ctcaaaaagc aggtggaaaa agccatgggg
     2521 aacgaaatgt ctgctcatca gtacctggca aaccttgttg gcctggcaga aaatataacc
     2581 caggaacgtg acagtcttat gtgtttggca aaatgtttag aaagtgagaa ggatggagtg
     2641 cttaataaag tcataaaaag caacattcgc ctgggaaagt tagaggaaaa agtcaagggc
     2701 tacaagaagc aggcagcact gaagctgggg gacatcagtc accgtctgct ggagcagcag
     2761 gaggacttcg ccggcaagac agcccagtac cggcaggaga tgcggcacct gcaccaggtg
     2821 ctgaaggaca agcaggaggt gctggaccag gcgctgcagc agaacagaga aatggaaggt
     2881 gaacttgaag ttatttggga atctaccttc agggaaaacc gaagaatccg agaacttctc
     2941 caggacacac tcacgaggac aggcgtgcag gacaacccca gagctctggt tgcccccagc
     3001 ctcaatggcg tctctcaggc agacctgctg gacggctgcg atgtctgctc ctatgacctg
     3061 aagtctcatg cccccacctg ctagggatcc gcggccgcga ccctaggccg acgcgtccgg
     3121 ttaaccggga tcgagatcct gcagaaccac gagtctggtt gcattctaga taattctacc
     3181 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3241 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3301 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     3361 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     3421 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     3481 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     3541 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     3601 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     3661 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     3721 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     3781 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     3841 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     3901 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     3961 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4021 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4081 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4141 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4201 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4261 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac
     4321 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     4381 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     4441 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4501 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4561 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4621 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4681 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     4741 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4801 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4861 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     4921 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     4981 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5041 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5101 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5161 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     5221 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     5281 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     5341 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     5401 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     5461 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     5521 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     5581 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5641 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5701 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     5761 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     5821 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     5881 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5941 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6001 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6061 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6121 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6181 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6241 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6301 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6361 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6421 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6481 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6541 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6601 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6661 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6721 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6781 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6841 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6901 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6961 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7021 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7081 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7141 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7201 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7261 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7321 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7381 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7441 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7501 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7561 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7621 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7681 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7741 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7801 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7861 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7921 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7981 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8041 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8101 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8161 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     8221 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     8281 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     8341 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     8401 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     8461 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     8521 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     8581 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     8641 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     8701 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     8761 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     8821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9001 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     9061 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9121 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9181 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9241 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     9301 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     9361 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     9421 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     9481 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     9541 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     9601 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     9661 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     9721 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     9781 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     9841 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9901 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9961 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10021 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10081 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10141 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10201 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10261 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    10321 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg cctttaaaag
    10381 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    10441 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    10501 cagggacagc agagatccag tttatcgata agcttgggag ttccgcgtta cataacttac
    10561 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    10621 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    10681 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    10741 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    10801 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    10861 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    10921 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    10981 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    11041 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
    11101 tgacctccat agaagacacc gactctacta gaggatcgct agcgctaccg gactcagatc
    11161 tcgac
//
