LOCUS       sequence-139054-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Violacein pathway (vioABECD) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux 
            redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC-3A2-vioD
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..171
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(210..228)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      224..271
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    324..348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             363..385
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atggtcacgg gtgatccgaa cgttcacgcc tctgtaagag actttcctaa ttttaatacg
       61 ctgccgatac ctaacacgat ttttgatgta acaagtctct ctcataggaa ttgactcgag
      121 tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc gtctactagt
      181 cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa taactagcat aaccccttgg
      241 ggcctctaaa cgggtcttga ggggtttttt gctaggatcg agatcgatct cgatcccgcg
      301 aaattaatac gactcactac ccgggaattg tgagcggata acaattcccc tctagaaata
      361 attttgttta actttaagaa ggagatatac atatgagtaa aataattatt gttggtggtg
      421 ggttggcagg cagcctgacg gcaatctatc tagcgaaaca agggcacgaa gttcacatta
      481 tagaaaaacg tggaaatcct ttagaaagtc aggcggatta tattgatcaa gttagcgcgc
      541 gtgcgattgg tgtcagtatg acagtacgag gaattgaagc cgttttagca gccggtattc
      601 ccttggacga attgcaggcg tgcggcgttg aagtttcagg tatggcgttt tttatcaaag
      661 gtc
//
