LOCUS       sequence-139057-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagcctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg gcgccacaag aacaagcagt ttctcaggtg atggagaaac
      181 agagcaacaa caacaacagt gacgtcattt tccgatcaaa gttaccggat atttacatcc
      241 cgaaccacct atctctccac gactacatct tccaaaacat ctccgaattc gccactaagc
      301 cttgcctaat caacggacca accggccacg tgtacactta ctccgacgtc cacgtcatct
      361 cccgccaaat cgccgccaat tttcacaaac tcggcgttaa ccaaaacgac gtcgtcatgc
      421 tcctcctccc aaactgtccc gaattcgtcc tctctttcct cgccgcctcc ttccgcggcg
      481 caaccgccac cgccgcaaac cctttcttca ctccggcgga gatagctaaa caagccaaag
      541 cctccaacac caaactcata atcaccgaag ctcgttacgt cgacaaaatc aaaccacttc
      601 aaaacgacga cggagtagtc atcgtctgca tcgacgacaa cgaatccgtg ccaatccctg
      661 aaggctgcct ccgcttcacc gagttgactc agtcgacaac cgaggcatca gaagtcatcg
      721 actcggtgga gatttcaccg gacgacgtgg tggcactacc ttactcctct ggcacgacgg
//
