LOCUS       sequence-139058-         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatggc gccacaagaa caagcagttt ctcaggtgat ggagaaacag
      181 agcaacaaca acaacagtga cgtcattttc cgatcaaagt taccggatat ttacatcccg
      241 aaccacctat ctctccacga ctacatcttc caaaacatct ccgaattcgc cactaagcct
      301 tgcctaatca acggaccaac cggccacgtg tacacttact ccgacgtcca cgtcatctcc
      361 cgccaaatcg ccgccaattt tcacaaactc ggcgttaacc aaaacgacgt cgtcatgctc
      421 ctcctcccaa actgtcccga attcgtcctc tctttcctcg ccgcctcctt ccgcggcgca
      481 accgccaccg ccgcaaaccc tttcttcact ccggcggaga tagctaaaca agccaaagcc
      541 tccaacacca aactcataat caccgaagct cgttacgtcg acaaaatcaa accacttcaa
      601 aacgacgacg gagtagtcat cgtctgcatc gacgacaacg aatccgtgcc aatccctgaa
      661 ggctgcctcc gcttcaccga gttgactcag tcgacaaccg aggcatcaga agtcatcgac
      721 tcggtggaga tttcaccgga cgacgtggtg gcactacctt actcctctgg cacgacgg
//
