LOCUS       sequence-139059-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gggagactgt gtagcaccca aagaagacct tattttccga
      181 tcgaaactcc ctgatattta catcccgaaa caccttccgt tacatactta ttgtttcgaa
      241 aacatctcga aagttggcga caagtcctgt ttaataaatg gcgctacagg cgaaacgttc
      301 acttattccc aagttgagct cctttccagg aaagttgcat cagggttaaa caaactcggc
      361 attcaacagg gcgataccat catgcttttg ctccctaact cccctgagta ttttttcgct
      421 ttcttaggcg catcgtatcg tggtgcaatt tctactatgg ccaatccgtt tttcacttct
      481 gctgaggtga tcaaacagct caaagcatcc caagctaagc tcataattac gcaagcttgt
      541 tacgtagaca aagtgaaaga ctacgcagca gagaaaaata tacagatcat ttgcatcgat
      601 gatgctcctc aggattgttt acatttctcc aaacttatgg aagctgatga atcagaaatg
      661 cctgaggttg tgatcaattc agacgatgtc gtcgcgttac cttactcatc gggtactaca
      721 ggactaccga aaggtgttat gttgacacac aaaggacttg ttactagcgt ggcacaacaa
      781 gttgatggag acaatccgaa tttatatatg catagcgagg atgtgatgat ctgcatattg
      841 cct
//
