LOCUS       sequence-139060-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagcctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
       61 actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg ggagactgtg tagcacccaa agaagacctt attttccgat
      181 cgaaactccc tgatatttac atcccgaaac accttccgtt acatacttat tgtttcgaaa
      241 acatctcgaa agttggcgac aagtcctgtt taataaatgg cgctacaggc gaaacgttca
      301 cttattccca agttgagctc ctttccagga aagttgcatc agggttaaac aaactcggca
      361 ttcaacaggg cgataccatc atgcttttgc tccctaactc ccctgagtat tttttcgctt
      421 tcttaggcgc atcgtatcgt ggtgcaattt ctactatggc caatccgttt ttcacttctg
      481 ctgaggtgat caaacagctc aaagcatccc aagctaagct cataattacg caagcttgtt
      541 acgtagacaa agtgaaagac tacgcagcag agaaaaatat acagatcatt tgcatcgatg
      601 atgctcctca ggattgttta catttctcca aacttatgga agctgatgaa tcagaaatgc
      661 ctgaggttgt gatcaattca gacgatgtcg tcgcgttacc ttactcatcg ggtactacag
      721 gactaccgaa aggtgttatg ttgacacaca aaggacttgt tactagcgtg gcacaacaag
      781 ttgatggaga caatccgaat ttatatatgc atagcgagga tgtgatgatc tgcatattgc
      841 ctt
//
