LOCUS       sequence-139071-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in Mammalian Cells, Cas9 Nuclease to analize 
            translocations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, 
            Sfeir A
  TITLE     Mammalian polymerase theta promotes alternative NHEJ and suppresses 
            recombination.
  JOURNAL   Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 
            2015 Feb 2.
  PUBMED    25642960
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature14157"; journalName: "Nature"; date: "2015-02-12- 
            12"; volume: "518"; issue: "7538"; pages: "254-7"
COMMENT     Sequence Label: pX330(puro) Rosa26-H3F3B
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(210..285)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(365..384)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        complement(643..718)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactattgac gtcaatgggc
       61 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgggtacct
      121 ctagagccat ttgtctgcag aattggcgca cgcgctaaaa acggactagc cttattttaa
      181 cttgctattt ctagctctaa aacaaaaaag caccgactcg gtgccacttt ttcaagttga
      241 taacggacta gccttatttt aacttgctat ttctagctct aaaactcttc tagaaagact
      301 ggagtcggtg tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca
      361 agttacggta agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc
      421 aagaaattat tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa
      481 attaattcca attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat
      541 gggaaaggta cctctagagc catttgtctg cagaattggc gcacgcgcta aaaacggact
      601 agccttattt taacttgcta tttctagctc taaaacaaaa aagcaccgac tcggtgccac
      661 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacgt
      721 tggctcgccg gatacgggcg gtgtttcgtc ctttccacaa
//
