LOCUS       sequence-139078-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat taatacgact
       61 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gattagtatt gaaacgcaaa acccggatgt tagcaacctg
      181 gacacctcgc actctattcc gaaaatggca aaccgtattg atgaccatgt gtttcgttct
      241 aaactgccgg aaattccgat cagtaaccat ctgccgctgc acacgtattg cttcgaaaat
      301 tactcgcagt ttgcagaccg tccgtgtctg attgttggct cgacgaacaa aacctatagc
      361 ttcgctgaaa cccatctgat ctctcgcaaa gtgggcgcag gttttgctca cctgggtctg
      421 aaacagggcg atgtggttat gattctgctg caaaattgcg cggaatttgc cttcagcttt
      481 ctgggtgcgt ctatggttgg cgccgtcacc acgaccgcaa acccgttcta cacgtccgcg
      541 gaaatcttca aacagctgaa cgcatcaaaa gctaaaatcg tcgtgaccca ggcgcaatat
      601 gtggataaac tgcgcgacta cccggatggt caagttgcca aaattggcga aggtttcacg
      661 gtcattacca tcgatgaccc gccggaaaac tgtatgcatt ttagtgttgt ctccgaagcg
      721 aacgaaagcg aactgccgga agtctcaatt aattcggatg acccggtggc c
//
