LOCUS       sequence-139080-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 acgatgcgtc cggcgtagcc taggatcgag atcgatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atggcgccac aagaacaagc agtttctcag gtgatggaga
      181 aacagagcaa caacaacaac agtgacgtca ttttccgatc aaagttaccg gatatttaca
      241 tcccgaacca cctatctctc cacgactaca tcttccaaaa catctccgaa ttcgccacta
      301 agccttgcct aatcaacgga ccaaccggcc acgtgtacac ttactccgac gtccacgtca
      361 tctcccgcca aatcgccgcc aattttcaca aactcggcgt taaccaaaac gacgtcgtca
      421 tgctcctcct cccaaactgt cccgaattcg tcctctcttt cctcgccgcc tccttccgcg
      481 gcgcaaccgc caccgccgca aaccctttct tcactccggc ggagatagct aaacaagcca
      541 aagcctccaa caccaaactc ataatcaccg aagctcgtta cgtcgacaaa atcaaaccac
      601 ttcaaaacga cgacggagta gtcatcgtct gcatcgacga caacgaatcc gtgccaatcc
      661 ctgaaggctg cctccgcttc accgagttga ctcagtcgac aaccgaggca tcagaagtca
      721 tcgactcggt ggagatttca ccggacgacg tggtggcact accttactcc tctggcacga
      781 cgggattacc aa
//
