LOCUS       sequence-139083-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 3F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-3F2-mCherry
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat taatacgact
       61 cactaacgtg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat ggtttcaaaa ggcgaagaag acaacatggc gattatcaag
      181 gaatttatgc gtttcaaggt ccacatggaa ggcagcgtca atggtcacga atttgaaatt
      241 gaaggcgaag gtgaaggccg tccgtatgaa ggcacccaga cggcaaaact gaaggtcacc
      301 aaaggcggtc cgctgccgtt tgcttgggat attctgtcac cgcaattcat gtatggttcg
      361 aaagcgtacg ttaagcatcc ggccgatatc ccggactatc tgaaactgtc ctttccggaa
      421 ggcttcaaat gggaacgtgt tatgaacttc gaagatggcg gtgtggttac cgtcacgcag
      481 gatagctctc tgcaagacgg tgaatttatt tataaagtga agctgcgcgg caccaatttc
      541 ccgagcgatg gtccggttat gcagaaaaag acgatgggct gggaagcgag ttccgaacgt
      601 atgtacccgg aagacggtgc cctgaaaggc gaaatcaagc agcgcctgaa actgaaggat
      661 ggcggtcact atgacgcaga agtgaaaacc acgtacaagg ctaaaaagcc ggtccaactg
      721 ccgggtgcat acaacgtgaa catcaagctg gatatcacca gccataacga agactatacg
      781 atcgttgaac agtacgaacg tgcagaaggc cgccactcta ccggcggtat ggatgaactg
      841 tacaa
//
