LOCUS       sequence-139089-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 4F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-4F2-mCherry
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactactta ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tggtttcaaa aggcgaagaa gacaacatgg cgattatcaa
      181 ggaatttatg cgtttcaagg tccacatgga aggcagcgtc aatggtcacg aatttgaaat
      241 tgaaggcgaa ggtgaaggcc gtccgtatga aggcacccag acggcaaaac tgaaggtcac
      301 caaaggcggt ccgctgccgt ttgcttggga tattctgtca ccgcaattca tgtatggttc
      361 gaaagcgtac gttaagcatc cggccgatat cccggactat ctgaaactgt cctttccgga
      421 aggcttcaaa tgggaacgtg ttatgaactt cgaagatggc ggtgtggtta ccgtcacgca
      481 ggatagctct ctgcaagacg gtgaatttat ttataaagtg aagctgcgcg gcaccaattt
      541 cccgagcgat ggtccggtta tgcagaaaaa gacgatgggc tgggaagcga gttccgaacg
      601 tatgtacccg gaagacggtg ccctgaaagg cgaaatcaag cagcgcctga aactgaagga
      661 tggcggtcac tatgacgcag aagtgaaaac cacgtacaag gctaaaaagc cggtccaact
      721 gccgggtgca tacaacgtga acatcaagct ggatatcacc agccataacg aagactatac
      781 gatcgttgaa cagtacgaac gtgcagaagg ccgccactct accggcggta tggatgaact
      841 gtacaa
//
