LOCUS       sequence-139091-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 1E4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-1E4-mCherry
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             108..130
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgcgtccgg cgtagcctag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
       61 actatacagg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt
      121 aagaaggaga tatacatatg gtttcaaaag gcgaagaaga caacatggcg attatcaagg
      181 aatttatgcg tttcaaggtc cacatggaag gcagcgtcaa tggtcacgaa tttgaaattg
      241 aaggcgaagg tgaaggccgt ccgtatgaag gcacccagac ggcaaaactg aaggtcacca
      301 aaggcggtcc gctgccgttt gcttgggata ttctgtcacc gcaattcatg tatggttcga
      361 aagcgtacgt taagcatccg gccgatatcc cggactatct gaaactgtcc tttccggaag
      421 gcttcaaatg ggaacgtgtt atgaacttcg aagatggcgg tgtggttacc gtcacgcagg
      481 atagctctct gcaagacggt gaatttattt ataaagtgaa gctgcgcggc accaatttcc
      541 cgagcgatgg tccggttatg cagaaaaaga cgatgggctg ggaagcgagt tccgaacgta
      601 tgtacccgga agacggtgcc ctgaaaggcg aaatcaagca gcgcctgaaa ctgaaggatg
      661 gcggtcacta tgacgcagaa gtgaaaacca cgtacaaggc taaaaagccg gtccaactgc
      721 cgggtgcata caacgtgaac atcaagctgg atatcaccag ccataacgaa gactatacga
      781 tcgttgaaca gtacgaacgt gcagaaggcc gccactctac cggcgg
//
