LOCUS       sequence-139094-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variant G6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tatttcggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgtt aaatttagag cattcagttt caattgtcgg agctggaatt
      181 tctgggatag tgtgtgcaat tacattagct cgttcaaggg cattagaagg ttacgcaata
      241 gatgtctatg aacaaagccc ccgcgttggt ggacggatcc aaacaaactt gttgagcaat
      301 cattacatcg atttaggtgc cgctcgtttt tgttcacaat tgcatggtaa tgtatatgat
      361 ctagtcaaga ggtttgaact cgaatttgaa ccgtttcctt ttactcaact tagatttact
      421 cagccaattc aaaaagaact caagactatt ttgtctaatc taaaaccgtt aatcgatgaa
      481 cataaagaag attcttttgt cgaatttgtt acgagctatt tgggtaaaga aaaagcaaaa
      541 gcagtaatct tagcactggg ctacgactca ctctctatgc caataatttc tccacatatt
      601 gcttacgata tcattgaaaa acacccagaa actcaatgtt tcactgaaaa ttgtgggaat
      661 gaatggttta gtttgaaaga gggattggcg tcgttgatca aggcgctata tgaagaggcg
      721 ttaaaactcg ggattaagtt tcattttgaa tctacattag ttcaa
//
