LOCUS       sequence-139096-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Violacein pathway (vioABECD) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux 
            redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC-3A2-vioD
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactatttc ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgttaaattt agagcattca gtttcaattg tcggagctgg
      181 aatttctggg atagtgtgtg caattacatt agctcgttca agggcattag aaggttacgc
      241 aatagatgtc tatgaacaaa gcccccgcgt tggtggacgg atccaaacaa acttgttgag
      301 caatcattac atcgatttag gtgccgctcg tttttgttca caattgcatg gtaatgtata
      361 tgatctagtc aagaggtttg aactcgaatt tgaaccgttt ccttttactc aacttagatt
      421 tactcagcca attcaaaaag aactcaagac tattttgtct aatctaaaac cgttaatcga
      481 tgaacataaa gaagattctt ttgtcgaatt tgttacgagc tatttgggta aagaaaaagc
      541 aaaagcagta atcttagcac tgggctacga ctcactctct atgccaataa tttctccaca
      601 tattgcttac gatatcattg aaaaacaccc agaaactcaa tgtttcactg aaaattgtgg
      661 gaatgaatgg tttagtttga aagagggatt ggcgtcgttg atcaaggcgc tatatgaaga
      721 ggcgtt
//
