LOCUS       sequence-139098-         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant C4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-C4
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   370..405
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   436..471
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(472..603)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
       61 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
      121 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
      181 gaatggacga tcacactact cttcttttgc ctattataac atgaaaacgc tttaacagaa
      241 agaataggaa ggtatccgac tgctggtatt aaccctcttt ctcaagttat catcggcaat
      301 gttgaatgga gtccattcaa aacagcatag ctctaaaaca tggaactcaa caagtctcag
      361 tgtgctgaag ttttgggacc attcaaaaca gcatagctct aaaactgata gtgagtcgta
      421 ttaatttcgc gggatgtttt gggaccattc aaaacagcat agctctaaaa cctcgtagac
      481 tatttttgtc taaaaaattt cgtaatcgca ctatttgtct cagctagact tcagtcttga
      541 aaagcccctg tattactgca tttattaaga gtattatacc atatttttag ttattaagaa
      601 ataatcttca tctaaaatat acttcagtca cctcctagct gactcaaatc aatgcgtgtt
      661 tcataaagac cagtgatgga ttgatggata agagtggcat ctaaaacttc tt
//
