LOCUS       sequence-139106-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 4F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-4F2
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   382..417
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   448..483
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(484..615)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 ccggcgtaga ggatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct
       61 ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga
      121 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg
      181 gcgatgctgt cggaatggac gatcacacta ctcttctttt gcctattata acatgaaaac
      241 gctttaacag aaagaatagg aaggtatccg actgctggta ttaaccctct ttctcaagtt
      301 atcatcggca atgttgaatg gagtccattc aaaacagcat agctctaaaa catggaactc
      361 aacaagtctc agtgtgctga agttttggga ccattcaaaa cagcatagct ctaaaacaag
      421 tagtgagtcg tattaatttc gcgggatgtt ttgggaccat tcaaaacagc atagctctaa
      481 aacctcgtag actatttttg tctaaaaaat ttcgtaatcg cactatttgt ctcagctaga
      541 cttcagtctt gaaaagcccc tgtattactg catttattaa gagtattata ccatattttt
      601 agttattaag aaataatctt catctaaaat atacttc
//
