LOCUS       sequence-139114-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 4A6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-4A6
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   385..420
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   451..486
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(487..618)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 cgtccggcgt agaggatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
       61 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
      121 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga
      181 ctggcgatgc tgtcggaatg gacgatcaca ctactcttct tttgcctatt ataacatgaa
      241 aacgctttaa cagaaagaat aggaaggtat ccgactgctg gtattaaccc tctttctcaa
      301 gttatcatcg gcaatgttga atggagtcca ttcaaaacag catagctcta aaacatggaa
      361 ctcaacaagt ctcagtgtgc tgaagttttg ggaccattca aaacagcata gctctaaaac
      421 gggtagtgag tcgtattaat ttcgcgggat gttttgggac cattcaaaac agcatagctc
      481 taaaacctcg tagactattt ttgtctaaaa aatttcgtaa tcgcactatt tgtctcagct
      541 agacttcagt cttgaaaagc ccctgtatta ctgcatttat taagagtatt ataccatatt
      601 tttagttatt aagaaataat cttcatctaa aatatacttc agtcacctcc tagctgactc
      661 aaatcaatgc gtgtttcata aagaccagtg atggattgat ggataagagt ggcatctaaa
      721 acttcttttg tagacgtata tcgt
//
