LOCUS       sequence-139117-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 1E4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-1E4
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   390..425
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   456..491
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(492..623)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga
       61 tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg
      121 ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat
      181 agtgactggc gatgctgtcg gaatggacga tcacactact cttcttttgc ctattataac
      241 atgaaaacgc tttaacagaa agaataggaa ggtatccgac tgctggtatt aaccctcttt
      301 ctcaagttat catcggcaat gttgaatgga gtccattcaa aacagcatag ctctaaaaca
      361 tggaactcaa caagtctcag tgtgctgaag ttttgggacc attcaaaaca gcatagctct
      421 aaaacgtata gtgagtcgta ttaatttcgc gggatgtttt gggaccattc aaaacagcat
      481 agctctaaaa cctcgtagac tatttttgtc taaaaaattt cgtaatcgca ctatttgtct
      541 cagctagact tcagtcttga aaagcccctg tattactgca tttattaaga gtattatacc
      601 atatttttag ttattaagaa ataatcttca tctaaaatat acttcagtca cctcctagct
      661 gactca
//
