LOCUS       sequence-139119-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant G6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-G6
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   219..254
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   285..320
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     misc_feature    complement(321..452)
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
ORIGIN
        1 gcacgccata gtgactggcg atgctgtcgg aatggacgat cacactactc ttcttttgcc
       61 tattataaca tgaaaacgct ttaacagaaa gaataggaag gtatccgact gctggtatta
      121 accctctttc tcaagttatc atcggcaatg ttgaatggag tccattcaaa acagcatagc
      181 tctaaaacat ggaactcaac aagtctcagt gtgctgaagt tttgggacca ttcaaaacag
      241 catagctcta aaacaaatag tgagtcgtat taatttcgcg ggatgttttg ggaccattca
      301 aaacagcata gctctaaaac ctcgtagact atttttgtct aaaaaatttc gtaatcgcac
      361 tatttgtctc agctagactt cagtcttgaa aagcccctgt attactgcat ttattaagag
      421 tattatacca tatttttagt tattaagaaa taatcttcat ctaaaatata cttcagtcac
      481 ctcctagctg actcaaatca atgcgtgttt cataaagacc agtgatggat tgatggataa
      541 gagtggcatc taa
//
