LOCUS       sequence-139126-        5120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Kumar MS, Erkeland SJ, Pester RE, Chen CY, Ebert MS, Sharp PA, Jacks
            T
  TITLE     Suppression of non-small cell lung tumor development by the let-7 
            microRNA family.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3903-8. doi: 
            10.1073/pnas.0712321105
  PUBMED    18308936
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2008-03-11- 11"; 
            volume: "105"; issue: "10"; pages: "3903-8"
COMMENT     Sequence Label: pBabe zeo HMGA2
FEATURES             Location/Qualifiers
     source          1..5120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(760..1134)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1144..1473)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1158..1293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1458..1478
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(1845..2261)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(1861..1877)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(1893..1915)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    2462..2661
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             2726..3195
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        3226..3330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3331..3399,3400..4191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3549..3568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4840..4859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5093..5110
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgatcggt cagtcctgct cctcggccac
      781 gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc acggctgctc
      841 gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca cgacctccga
      901 ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg tgttgtccgg
      961 caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc
     1021 gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga actcgaccgc
     1081 tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg ccatggtaag
     1141 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     1201 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     1261 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     1321 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     1381 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     1441 gcctggggac tttccacacc ctaactgaca cacattccac agggtcgacc actgtgctgg
     1501 cgaattccta atcctcctct gcggactctt gcgaggatgt ctcttcagtc tcctgagcag
     1561 gcttcttctg aacgacttgt tgtggccatt tcctaggtct gcctcttggc cgtttttctc
     1621 caatggtctc tgctttcttc tgggctgctt tagaggggct cttgtttttg ctgcctttgg
     1681 gtcttcctct gggtctctta ggagagggct cacaggttgg ctcttgctgc tgcttcctgg
     1741 gtcggccgcg tcctcgcttc tgtggcaccg gggcggcagg ttgtccctgg gctgatgtgg
     1801 acggctgccc ggcgccctca ccgcgtgcgc tcatggatcc ggccggcgcc tagagaagga
     1861 gtgagggctg gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga
     1921 gacggggcgg atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag
     1981 ggaggggggt caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt
     2041 ccatgcgggc caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag
     2101 gtgccgtctc gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc
     2161 caacgtctct tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag
     2221 tgacctaagg tcaaacttaa gggagtggta acagtctggc cctaattttc agacaaatac
     2281 agaaacacag tcagacagag acaacacaga acgatgctgc agcgctgcag cagacaagac
     2341 gcgcggcttc ggttccaaac cgaaagcaaa aattcagacg gaggcgggaa ctgttttagg
     2401 ttctcgtctc ctaccagaac cacatatcct gacggggtcg gattccacat cgactccctt
     2461 cctcaggtcg ggccacaaaa acggccccca aagtccctgg gacgtctccc agggttgcgg
     2521 ccgggtgttc agaactcgtc agttccacca cgggtccgcc agatacagag ctagttagct
     2581 aactagtacc gacgcaggcg cataaaatca gtcatagaca ctagacaatc ggacagacac
     2641 agataagttg ctggccagct tgcctcccgg tggtgggtcg gtggtccctg ggcaggggtc
     2701 tcccgatccc ggacgagccc ccaaatgaaa gacccccgct gacgggtagt caatcactca
     2761 gaggagaccc tcccaaggaa cagcgagacc acaagtcgga tgcaactgca agagggttta
     2821 ttggatacac gggtacccgg gcgactcagt caatcggagg actggcgccc cgagtgaggg
     2881 gttgtgggct cttttattga gctcggggag cagaagcgcg cgaacagaag cgagaagcga
     2941 actgattggt tagttcaaat aaggcacagg gtcatttcag gtccttgggg caccctggaa
     3001 acatctgatg gttctctaga aactgctgag ggctggaccg catctgggga ccatctgttc
     3061 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     3121 ctgttccatc tgttcttggc cctgagccgg ggcaggaact gcttaccaca gatatcctgt
     3181 ttggcccata ttcaggctgc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3241 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3301 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3361 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3421 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3481 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3541 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     3601 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3661 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3721 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3781 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3841 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3901 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3961 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4021 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4081 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4141 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4201 caagtttact catatatact ttagattgat ttgcggccgc aaaaggatct aggtgaagat
     4261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4981 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5041 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5101 agtgagcgag gaagcggaag
//
