LOCUS       sequence-139138-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             623..645
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    660..684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(684..703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(685..703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgagtatca tttggcccac ggaaagtgac cgcggtgatt tctgaacaaa caacaaggac
       61 tcgagcgccc ttgttgtttt cagccaagtc cttggctaac cgaagaaccg tgccaccagc
      121 aaagcaacct tgttggtaca tcataagcct cttgaccgat ggacgaagcc caagtagctt
      181 agtgagttga tagtcacacc caggcatgtc cacaccactg gttgtgcaaa agaccaaatg
      241 ggtaattttg gacttgggct ggccccattc cttgatggcc ttttgagctg cctctttgcc
      301 aagtttgggc acttcaacca ccactatgtc ttgcctagca tcaagagaag gtgccatgta
      361 ttcacacata ctaggattct ctttcaagat ttcctctgtt aagtgcatgt accttttctt
      421 aatcattgat ttttcacaca tgcgcttaaa tttctcctta agatcagtct tgtgctcact
      481 gttagtgata cgaaaataaa aatcagggta agtgctttga tcaacacagt ttgaaggtgt
      541 ggctgttcca atggccatga cagtggctgg accttcagca cgttgtgcct tacgatactc
      601 ctcgactgtc accatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg
      661 gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga
      721 tcgatctcga tcctagcaa
//
