LOCUS       sequence-139140-        4101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4101)
  TITLE     Kirschner Lab Plasmids.
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2P+
FEATURES             Location/Qualifiers
     source          1..4101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    373..507
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(410..429)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     464..483
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     512..531
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        512..530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(607..625)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(608..625)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(660..1644)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      1715..2170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1802..1821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2012..2033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2196..2300
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2301..2369,2370..3161)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2519..2538)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3332..3920
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3821..3840
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4074..4091
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gcccaatgca ttggcgccgc
      301 ggccgcgaat taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
      361 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
      421 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
      481 atcaatgtat cttatcatgt ctggatctac gtaatacgac tcactatagg gctgcagaat
      541 ctagaggctc gagaggcctt gaattcgaat cgatgggatc ctgcaaaaag aacaagtagc
      601 ttgtattcta tagtgtcacc taaatcaagc ttgctccgac gtccccaggc agaatggcgg
      661 ttccctaaac gagcattgct tatatagacc tcccattagg cacgcctacc gcccatttac
      721 gtcaatggaa cgcccatttg cgtcattgcc cctccccatt gacgtcaatg gggatgtact
      781 tggcagccat cgcgggccat ttaccgccat tgacgtcaat gggagtactg ccaatgtacc
      841 ctggcgtact tccaatagta atgtacttgc caagttacta ttaatagata ttgatgtact
      901 gccaagtggg ccatttaccg tcattgacgt caataggggg cgtgagaacg gatatgaatg
      961 ggcaatgagc catcccattg acgtcaatgg tgggtggtcc tattgacgtc aatgggcatt
     1021 gagccaggcg ggccatttac cgtaattgac gtcaatgggg gaggcgccat atacgtcaat
     1081 aggaccgccc atatgacgtc aataggaaag accatatata gagaccattg acgtcaatgg
     1141 gggagtggct atgggcggta ttaggaagcc ccatatatgg tatatgggac cgcccattgg
     1201 gaggggctat ctacgtcaat aggaaaaccc atatatggaa tactatatgg catagggcca
     1261 atacatagta ttgaacctgg ccaatagcca tattggcata gggccatatt ggatattgcc
     1321 tatatattga tcctggcata tagccaatat ggccgccatt attggcacca tgccaattca
     1381 atatggcgga cttggcacag ggccaagacc cctcctcaaa cttggcaccg tgccaagtag
     1441 acccctcccc agttggcaca gtgccaggtc cgccatattg aattggcatg gtgccaagtc
     1501 cgccatattg aattggctgg ggtccagatc caccatattg aattggcacg agtccatata
     1561 cgccatattg aattggcaca ggtccagatc caccatattg aattggcatg agtccatata
     1621 cgccatattg aattggcggt cgactggcgt aatagcgaag aggcccgcac cgatcgccct
     1681 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     1741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     1801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     1861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     1921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     1981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2161 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2221 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2281 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2341 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2401 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2461 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2521 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2581 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2641 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2701 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2761 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2821 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2881 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2941 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3001 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3061 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3121 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3181 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3241 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3301 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3361 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3421 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3481 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3541 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3601 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3661 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3721 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3781 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3841 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3901 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3961 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4021 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4081 cagtgagcga ggaagcggaa g
//
