LOCUS       sequence-139157-        9137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1728..1772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1825..1872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1906..1924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1925..1949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1964..1986
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3185..3229
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3282..3329
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3363..3381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3382..3406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3421..3443
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4169..4216
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4260..4715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4557..4578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4830..5621,5622..5690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5453..5472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5691..5783)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5864..6452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6353..6372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6606..6623
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6638..6780
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6706..6725)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6866..6888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6882..7073)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7645..8727)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8689..8708
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8728..8805)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9011..9030
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9121..9137,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgggagac tgtgtagcac ccaaagaaga ccttattttc cgatcgaaac
      121 tccctgatat ttacatcccg aaacaccttc cgttacatac ttattgtttc gaaaacatct
      181 cgaaagttgg cgacaagtcc tgtttaataa atggcgctac aggcgaaacg ttcacttatt
      241 cccaagttga gctcctttcc aggaaagttg catcagggtt aaacaaactc ggcattcaac
      301 agggcgatac catcatgctt ttgctcccta actcccctga gtattttttc gctttcttag
      361 gcgcatcgta tcgtggtgca atttctacta tggccaatcc gtttttcact tctgctgagg
      421 tgatcaaaca gctcaaagca tcccaagcta agctcataat tacgcaagct tgttacgtag
      481 acaaagtgaa agactacgca gcagagaaaa atatacagat catttgcatc gatgatgctc
      541 ctcaggattg tttacatttc tccaaactta tggaagctga tgaatcagaa atgcctgagg
      601 ttgtgatcaa ttcagacgat gtcgtcgcgt taccttactc atcgggtact acaggactac
      661 cgaaaggtgt tatgttgaca cacaaaggac ttgttactag cgtggcacaa caagttgatg
      721 gagacaatcc gaatttatat atgcatagcg aggatgtgat gatctgcata ttgcctttgt
      781 ttcatattta ttcgcttaac gcggtgttgt gctgtggact cagagcaggg gtgacgatct
      841 tgattatgca gaaatttgat attgtgccat ttttggaact gatacagaaa tataaagtta
      901 caattggacc gtttgtgcca ccaattgtgt tggcaattgc gaaaagtcca gtggtggata
      961 aatatgactt gtcgtcggtg aggacggtta tgtctggagc tgctccgtta gggaaggagc
     1021 ttgaagatgc tgttagagct aagtttccta atgccaaact tggtcaggga tatggaatga
     1081 cagaggcagg gccagtttta gcaatgtgcc tggcgtttgc aaaggaacca tacgagatca
     1141 aatcgggtgc ctgtggaact gttgtgagga atgctgaaat gaaaattgtg gatcctgaga
     1201 ccaacgcctc tcttccacga aaccaacgcg gagagatttg cattcgaggt gaccaaatta
     1261 tgaaaggcta cctcaatgat cctgaatcaa caaggacaac aatagacgaa gaaggctggt
     1321 tgcacacagg agatataggc ttcattgacg acgatgatga gctatttatt gttgatagac
     1381 ttaaggaaat aatcaaatac aaaggcttcc aggttgcccc tgctgaactt gaagctctgc
     1441 tacttactca tcctaccatt tccgatgctg cagttgttcc catgatagat gagaaagcag
     1501 gagaggtgcc tgtggctttt gttgtgagaa caaacggttt caccaccact gaggaagaaa
     1561 tcaagcaatt cgtctcgaaa caggtggtgt tctacaagag aatatttcgt gtattttttg
     1621 ttgatgcaat tccgaaatca ccatctggaa agattcttcg aaaggacttg agagcaaaaa
     1681 tagcatccgg tgatcttccc aaataatagg gtaccctcga gtctggtaaa gaaaccgctg
     1741 ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta attaacctaa
     1801 actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1861 aggggttttt tgctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     1921 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     1981 aggagatata catatggtga cagtcgagga gtatcgtaag gcacaacgtg ctgaaggtcc
     2041 agccactgtc atggccattg gaacagccac accttcaaac tgtgttgatc aaagcactta
     2101 ccctgatttt tattttcgta tcactaacag tgagcacaag actgatctta aggagaaatt
     2161 taagcgcatg tgtgaaaaat caatgattaa gaaaaggtac atgcacttaa cagaggaaat
     2221 cttgaaagag aatcctagta tgtgtgaata catggcacct tctcttgatg ctaggcaaga
     2281 catagtggtg gttgaagtgc ccaaacttgg caaagaggca gctcaaaagg ccatcaagga
     2341 atggggccag cccaagtcca aaattaccca tttggtcttt tgcacaacca gtggtgtgga
     2401 catgcctggg tgtgactatc aactcactaa gctacttggg cttcgtccat cggtcaagag
     2461 gcttatgatg taccaacaag gttgctttgc tggtggcacg gttcttcggt tagccaagga
     2521 cttggctgaa aacaacaagg gcgctcgagt ccttgttgtt tgttcagaaa tcaccgcggt
     2581 cactttccgt gggccaaatg atactcattt ggatagttta gttggccaag cactttttgg
     2641 tgatggggca ggcgcgatca ttataggttc tgatccaatt ccaggggtcg aaaggccttt
     2701 gttcgagctc gtttcagcag cccaaactct tctcccagat agccatggtg ctattgatgg
     2761 ccatctccgt gaagttgggc ttacattcca cttactcaaa gatgttcctg ggctgatctc
     2821 aaaaaatatt gagaagagcc ttgaggaagc attcaaacct ttgggcattt ctgattggaa
     2881 ctctctattc tggattgctc atccaggtgg gcctgcaatt ttggaccaag ttgaaataaa
     2941 gttgggccta aagcccgaga aacttaaggc tacaaggaat gtgttaagta actatggtaa
     3001 catgtcaagt gcttgtgtac tgtttatttt ggatgaaatg agaaaggcct cagccaaaga
     3061 aggtttagga actactggtg aagggcttga gtggggtgtt ctttttggat ttgggcctgg
     3121 gctaacagtt gagactgttg tcctccacag tgttgctact taatagggta ccctcgagtc
     3181 tggtaaagaa accgctgctg cgaaatttga acgccagcac atggactcgt ctactagtcg
     3241 cagcttaatt aacctaaact gctgccaccg ctgagcaata actagcataa ccccttgggg
     3301 cctctaaacg ggtcttgagg ggttttttgc taggatcgag atcgatctcg atcccgcgaa
     3361 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
     3421 tttgtttaac tttaagaagg agatatacat atggctgcat caatcaccgc aatcactgtg
     3481 gagaaccttg aatacccagc ggtggttacc tctccggtca ccggcaaatc atatttcctc
     3541 ggtggcgctg gggagagagg attgaccatt gaaggaaact tcatcaagtt cactgccata
     3601 ggtgtttatt tggaagatat agcagtggct tcactagctg ccaaatggaa gggtaaatca
     3661 tctgaagagt tacttgagac ccttgacttt tacagagaca tcatctcagg tccctttgaa
     3721 aagttaatta gagggtcaaa gattagggaa ttgagtggtc ctgagtactc aaggaaggtt
     3781 atggagaact gtgtggcaca cttgaaatca gttggaactt atggagatgc agaagctgaa
     3841 gctatgcaaa aatttgctga agctttcaag cctgttaatt ttccacctgg tgcctctgtt
     3901 ttctacaggc aatcacctaa tggaatatta gggcttagtt tctctccgga tacaagtata
     3961 ccagaaaagg aggctgcact catagagaac aaggcagttt catcagcagt gttggagact
     4021 atgatcggcg agcacgctgt ttcccctgat cttaagcgct gtttagctgc aagattacct
     4081 gcgttgttga acgagggtgc tttcaagatt ggaaactgaa ctagtcgcag cttaattaac
     4141 ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     4201 cttgaggggt tttttgctag cgaaaggagg agtcgactat atccggattg gcgaatggga
     4261 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4321 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4381 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4441 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4501 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4561 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4621 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4681 cgcgaatttt aacaaaatat taacgtttac aatttctggc ggcacgatgg catgagatta
     4741 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4801 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4861 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4921 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4981 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5041 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5101 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5161 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5221 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5281 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5341 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5401 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5461 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5521 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5581 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5641 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5701 tttcaatcat gattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5761 atgtatttag aaaaataaac aaataggtca tgaccaaaat cccttaacgt gagttttcgt
     5821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5941 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6001 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6181 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     6601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     6661 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     6721 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     6781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     6841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     6901 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     6961 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     7021 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     7081 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     7141 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     7201 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     7261 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     7321 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     7381 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     7441 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     7501 cgggtcctca acgacaggag cacgatcatg ctagtcatgc cccgcgccca ccggaaggag
     7561 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     7621 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7681 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag
     7741 ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc
     7801 ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt
     7861 gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac
     7921 cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc
     7981 catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat
     8041 ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat
     8101 ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact
     8161 taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc
     8221 cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac
     8281 atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc
     8341 atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc
     8401 cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag
     8461 ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact
     8521 ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt
     8581 gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
     8641 gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc
     8701 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     8761 ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct
     8821 ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca
     8881 ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg
     8941 ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat
     9001 cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga
     9061 tgccggccac gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat
     9121 taatacgact cactata
//
