LOCUS       sequence-139162-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Zhang P, Medwig-Kinney TN, Goldstein B
  TITLE     Architecture of the cortical actomyosin network driving apical 
            constriction in C. elegans.
  JOURNAL   J Cell Biol. 2023 Sep 4;222(9):e202302102. doi: 
            10.1083/jcb.202302102. Epub 2023 Jun 23.
  PUBMED    37351566
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-09-4"; volume: "222(9):e202302102. doi"; pages: 
            " 10.1083/jcb.202302102. Epub 2023 Jun 23"
COMMENT     Sequence Label: pDD315 (mTurquoise2^SEC^2xHA)
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(82..106)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    459..492
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             511..537
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             538..564
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
       61 cgtccgaggg caaaggaata gacccagctt tcttgtacaa agtgggtcca attactcttc
      121 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
      181 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
      241 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
      301 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
      361 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
      421 atcatatgtt acgtttcagt ttatgaccgc aatttttaat aacttcgtat aatgtatact
      481 atacgaagtt attttcaggg agccggctct tatccatacg atgtcccaga ttacgcttac
      541 ccatatgacg ttccagacta tgccggagga ctagttcgac ctgcagactg gctgtgtata
      601 agggagcctg acatttagat tccccagaac ataaggttga tagcgttctt gatatcgttc
      661 tcacggtgag aaaggtcggc aacctcctct ccgatgacgg aaactggaac ggaagccatg
      721 tcggtggtca tcatacgcca agactcgtct ccgatgtgaa caact
//
