LOCUS       sequence-139168-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             637..681
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(720..738)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cgtggcctgg aaatcgaagg caaatttgtt aaattcaccg ctattggtgt ctatctggaa
       61 gaaaacgccg tgccgctgct ggcaggcaaa tggaaaggca aaaccgccgg tgaactgacg
      121 gaatctgtcg aatttttccg cgatgtggtt accggcccgt ttgaaaaatt catgaaagtg
      181 accatgatcc tgccgctgac gggtgcgcag tattcagaaa aagttgctga aaattgcatg
      241 gcgatttgga aatttttcgg catctacacc gatgcagaag ctaaagcgat tgaaaaattt
      301 acggaagtgt tcaaagacga aatttttccg ccgggcagct ctatcctgtt cacccaaagt
      361 tccggttcgc tgacgatttc attttcgaaa gatggcagca tcccgaaaga cggtgtcgcg
      421 gtgattgaaa acaatctgct gagcgaagcc gttctggaat ctatgatcgg taaaaacggc
      481 gtcagtccgg cggccaaaaa atccctggcc gaacgtctgt cagcactgct gaatgttgct
      541 tccgacaaaa tgaaaggcgg tggcggctca ggtggcggtg gctctggtgg cggtggttca
      601 ggcgtcaaag aaagtctggt gtgactcgag tctggtaaag aaaccgctgc tgcgaaattt
      661 gaacgccagc acatggactc gtctactagt cgcagcttaa ttaacctaaa ctgctgccac
      721 cgctgagcaa taactagcat aacc
//
