LOCUS       sequence-139169-        7689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SMCR8 HA tagged in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7689)
  TITLE     pcDNA3 HA C9ORF72 op
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCMV6 SMCR8 HA
FEATURES             Location/Qualifiers
     source          1..7689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3861..3887
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3903..3926
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3946..3962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3946..3962)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    3973..4595
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4172..4191)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     rep_origin      complement(4744..5332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4824..4843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5661..5708
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5733..5752
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5940..6734)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5940..6734)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6051..6070)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6661..6680
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6769..7126)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6783..6918
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6935..7126
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7083..7103
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(7128..7232)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7259..7687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7346..7365)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7556..7577
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gatcagcgcc cctgacgtag tggccttcac caaagaggaa gagtatgaag
     1081 aagagcctta caatgagccg gccctgcctg aggagtactc ggtgccgctc ttccccttcg
     1141 ccagtcaggg tgctaacccc tggtcaaaac tgtccggggc caagttttcg agggacttca
     1201 ttcttatttc cgagttctct gagcaggtgg gaccccaacc cttactgacc atccccaatg
     1261 acaccaaagt ttttggcact tttgatctca attacttctc cctgcgtatc atgtctgtgg
     1321 attaccaggc ttccttcgtg ggccatcctc ctggatctgc ctaccccaag ctgaacttcg
     1381 tggaggactc caaggtggtg ctgggagatt ctaaggaggg cgcctttgca tacgtgcacc
     1441 accttaccct atacgacctg gaggcccgtg gcttcgtgag gccgttttgc atggcttata
     1501 tctctgcaga ccagcataaa atcatgcagc agttccagga gctttcagcc gaattttcca
     1561 gagcttctga gtgcttgaag actggcaaca ggaaggcatt tgctggggaa cttgaaaaaa
     1621 agctgaaaga cttggattac accaggacag tgctacacac agaaacggag atccagaaga
     1681 aagccaacga caaaggcttt tactcatctc aggcaattga gaaagccaat gaactggcca
     1741 gtgtggagaa gtccatcatt gaacatcaag acctgctgaa gcagatccgc tcataccctc
     1801 atcggaagtt gaaggggcat gatttgtgtc ctggtgagat ggagcacatc caggatcagg
     1861 ccagccaggc atccactacc tctaaccctg atgagtctgc cgacacagac ctttacacct
     1921 gcagaccagc ctacacccca aaacttatca aagcaaagtc caccaagtgt tttgacaaga
     1981 agttgaagac cttggaggag ctctgtgaca ctgaatattt cacccagacc ctggctcagc
     2041 tcagccacat tgaacacatg ttcagaggag acctgtgtta cctcctgacc agtcagattg
     2101 atagagcact tctaaaacaa cagcatataa caaactttct ctttgaagac tttgtggagg
     2161 tcgatgacag gatggtggag aaacaagaaa gcataccctc taagcccagt caagacaggc
     2221 cgccttccag ttctctagaa gaatgcccaa ttcctaaagt gttaattagt gttggttctt
     2281 acaagtccag tgtggagtct gtgttgatca agatggagca ggaactggga gatgaggagt
     2341 acaaggaagt ggaagtgact gagttgagca gtttcgaccc ccaggaaaac ttggactacc
     2401 tggatatgga tatgaaaggg agtatcagca gtggtgaaag tattgaagtt ttgggcacgg
     2461 agaaatccac ctccgtgctt tctaaatctg acagccaggc aagcctcaca gtaccattga
     2521 gcccccaggt ggtccggagc aaagcagtca gccacaggac catcagcgag gacagtattg
     2581 aagtcctcag tacctgcccc tctgaggccc tcatccctga tgactttaag gccagctacc
     2641 caagtgccat taatgaagaa gaatcatatc cagatggcaa tgaaggagcc atccgcttcc
     2701 aggcaagcat cagtcctcca gaactgggtg agacagagga aggcagcata gaaaacaccc
     2761 catcacaaat agactcctcc tgctgtattg ggaaggagag cgatggtcag ttggtgctgc
     2821 cctccactcc agcccacaca cactctgacg aggatggggt ggtgagcagc cccccacagc
     2881 gccacaggca gaaggaccag gggttccgtg tagacttttc agtggaaaat gccaaccctt
     2941 cttcccgaga caacagttgt gaagggtttc ccgcttatga gctggacccg agccacctgc
     3001 tggctagccg ggacatcagt aagaccagcc tggacaacta ctcagacacc accagctacg
     3061 tgagcagtgt agcgtccacc agctcagaca ggatcccctc tgcttatcct gctggcctgt
     3121 cttccgatag gcataaaaag agggctggcc agaacgcctt aaaattcatc cgccagtacc
     3181 cctttgccca cccagccatc tactccctgc tcagtgggag gacacttgtg gtcctggggg
     3241 aagatgaggc catagtcagg aaactcgtga ctgcactggc tatctttgtc cccagctatg
     3301 gctgctacgc taagcccgtg aaacattggg cctcctcccc tttgcacatt atggattttc
     3361 agaagtggaa gcttattggc ttgcagagag tggcctcccc tgccggtgcc ggtaccctcc
     3421 atgccctgag ccgctacagc cgctacacga gcatcctgga ccttgacaac aaaaccctgc
     3481 gctgccccct ttacagaggc accctggtgc cccgcctggc agaccaccgc acacagatca
     3541 agcggggcag cacctactac ctgcatgtcc agagcatgct cacccagctc tgctccaagg
     3601 ccttcctcta caccttctgc caccacctgc acctgcctac ccacgacaag gagacagagg
     3661 agctggtagc cagccgccag atgagcttcc taaagctgac cctgggtctg gtgaatgagg
     3721 atgttagggt ggtccagtac ctggctgagc tgctgaagct gcactacatg caggaatctc
     3781 cagggaccag ccaccccatg ctcaggtttg actatgtccc cagctttttg tataaaatca
     3841 cgcgtacgcg gccgctcgag tacccatacg atgttccaga ttacgctaag taagatatcc
     3901 tggattacaa ggatgacgac gataaggttt aaacggccgg ccgcggtcat agctgtttcc
     3961 tgaacagatc ccgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
     4021 gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct
     4081 gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa
     4141 gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg gagtgcagtg
     4201 gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag
     4261 cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
     4321 tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
     4381 atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac tgctcccttc
     4441 cctgtccttc tgattttaaa ataactatac cagcaggagg acgtccagac acagcatagg
     4501 ctacctggcc atgcccaacc ggtgggacat ttgagttgct tgcttggcac tgtcctctca
     4561 tgcgttgggt ccactcagta gatgcctgtt gaattgggta cgcggccagc ggcgagcggt
     4621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     5041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     5101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     5161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     5221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5461 taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac
     5521 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     5581 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     5641 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt
     5701 tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct
     5761 ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc
     5821 ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa
     5881 atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     5941 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     6001 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     6061 gtagccaacg ctatgtcctg atagcgatcc gccacaccca gccggccaca gtcgatgaat
     6121 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     6181 acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg
     6241 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     6301 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     6361 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     6421 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     6481 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     6541 gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     6601 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     6661 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     6721 tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc
     6781 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     6841 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     6901 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     6961 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     7021 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     7081 acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact cttccttttt
     7141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7261 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7321 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7381 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7441 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7501 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7561 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7621 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7681 gcgaatttt
//
