LOCUS       sequence-139170-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             606..628
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    643..667
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(667..686)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(668..686)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cacggaaagt gaccgcggtg atttctgaac aaacaacaag gactcgagcg cccttgttgt
       61 tttcagccaa gtccttggct aaccgaagaa ccgtgccacc agcaaagcaa ccttgttggt
      121 acatcataag cctcttgacc gatggacgaa gcccaagtag cttagtgagt tgatagtcac
      181 acccaggcat gtccacacca ctggttgtgc aaaagaccaa atgggtaatt ttggacttgg
      241 gctggcccca ttccttgatg gccttttgag ctgcctcttt gccaagtttg ggcacttcaa
      301 ccaccactat gtcttgccta gcatcaagag aaggtgccat gtattcacac atactaggat
      361 tctctttcaa gatttcctct gttaagtgca tgtacctttt cttaatcatt gatttttcac
      421 acatgcgctt aaatttctcc ttaagatcag tcttgtgctc actgttagtg atacgaaaat
      481 aaaaatcagg gtaagtgctt tgatcaacac agtttgaagg tgtggctgtt ccaatggcca
      541 tgacagtggc tggaccttca gcacgttgtg ccttacgata ctcctcgact gtcaccatat
      601 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
      661 caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctagc
      721 aa
//
