LOCUS       sequence-139174-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             639..683
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(722..740)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aacgtggcct ggaaatcgaa ggcaaatttg ttaaattcac cgctattggt gtctatctgg
       61 aagaaaacgc cgtgccgctg ctggcaggca aatggaaagg caaaaccgcc ggtgaactga
      121 cggaatctgt cgaatttttc cgcgatgtgg ttaccggccc gtttgaaaaa ttcatgaaag
      181 tgaccatgat cctgccgctg acgggtgcgc agtattcaga aaaagttgct gaaaattgca
      241 tggcgatttg gaaatttttc ggcatctaca ccgatgcaga agctaaagcg attgaaaaat
      301 ttacggaagt gttcaaagac gaaatttttc cgccgggcag ctctatcctg ttcacccaaa
      361 gttccggttc gctgacgatt tcattttcga aagatggcag catcccgaaa gacggtgtcg
      421 cggtgattga aaacaatctg ctgagcgaag ccgttctgga atctatgatc ggtaaaaacg
      481 gcgtcagtcc ggcggccaaa aaatccctgg ccgaacgtct gtcagcactg ctgaatgttg
      541 cttccgacaa aatgaaaggc ggtggcggct caggtggcgg tggctctggt ggcggtggtt
      601 caggcgtcaa agaaagtctg gtgtgactcg agtctggtaa agaaaccgct gctgcgaaat
      661 ttgaacgcca gcacatggac tcgtctacta gtcgcagctt aattaaccta aactgctgcc
      721 accgctgagc aataactagc ataacc
//
