LOCUS       sequence-139176-         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             607..629
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    644..668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(668..687)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(669..687)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccacggaaag tgaccgcggt gatttctgaa caaacaacaa ggactcgagc gcccttgttg
       61 ttttcagcca agtccttggc taaccgaaga accgtgccac cagcaaagca accttgttgg
      121 tacatcataa gcctcttgac cgatggacga agcccaagta gcttagtgag ttgatagtca
      181 cacccaggca tgtccacacc actggttgtg caaaagacca aatgggtaat tttggacttg
      241 ggctggcccc attccttgat ggccttttga gctgcctctt tgccaagttt gggcacttca
      301 accaccacta tgtcttgcct agcatcaaga gaaggtgcca tgtattcaca catactagga
      361 ttctctttca agatttcctc tgttaagtgc atgtaccttt tcttaatcat tgatttttca
      421 cacatgcgct taaatttctc cttaagatca gtcttgtgct cactgttagt gatacgaaaa
      481 taaaaatcag ggtaagtgct ttgatcaaca cagtttgaag gtgtggctgt tccaatggcc
      541 atgacagtgg ctggaccttc agcacgttgt gccttacgat actcctcgac tgtcaccata
      601 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc
      661 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatcgatc tcgatcctag
      721 caa
//
