LOCUS       sequence-139181-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 4F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-4F2-mCherry
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtccatgtg ctggcgttca aatttcgcag cagcggtttc tttaccagac tcgagggtac
       61 cttatttgta cagttcatcc ataccgccgg tagagtggcg gccttctgca cgttcgtact
      121 gttcaacgat cgtatagtct tcgttatggc tggtgatatc cagcttgatg ttcacgttgt
      181 atgcacccgg cagttggacc ggctttttag ccttgtacgt ggttttcact tctgcgtcat
      241 agtgaccgcc atccttcagt ttcaggcgct gcttgatttc gcctttcagg gcaccgtctt
      301 ccgggtacat acgttcggaa ctcgcttccc agcccatcgt ctttttctgc ataaccggac
      361 catcgctcgg gaaattggtg ccgcgcagct tcactttata aataaattca ccgtcttgca
      421 gagagctatc ctgcgtgacg gtaaccacac cgccatcttc gaagttcata acacgttccc
      481 atttgaagcc ttccggaaag gacagtttca gatagtccgg gatatcggcc ggatgcttaa
      541 cgtacgcttt cgaaccatac atgaattgcg gtgacagaat atcccaagca aacggcagcg
      601 gaccgccttt ggtgaccttc agttttgccg tctgggtgcc ttcatacgga cggccttcac
      661 cttcgccttc aatttcaaat tcgtgaccat tgacgctgcc ttccatgtgg accttgaaac
      721 gcataa
//
