LOCUS       sequence-139183-        4480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacterial expression of Nano-lantern, a luminescent protein for 
            high-speed single-cell and whole body imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, 
            Yoshida Y, Matsuda T, Arai Y, Nagai T
  TITLE     Luminescent proteins for high-speed single-cell and whole-body 
            imaging.
  JOURNAL   Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248.
  PUBMED    23232392
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1262. doi"; pages: " 
            10.1038/ncomms2248"
COMMENT     Sequence Label: Nano-lantern/pRSETB
FEATURES             Location/Qualifiers
     source          1..4480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             167..184
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             188..220
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(224..232,233..247)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(254..256,257..259,260..772)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(299..320)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(560..579)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     907..928
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1936..1954)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1950..1997
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2094..2549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2181..2200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2391..2412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2575..2679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2680..2748,2749..3540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2898..2917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3711..4299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4200..4219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4453..4470
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggatct
       61 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa
      121 taattttgtt taactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca
      181 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      241 cgataaggat ccgatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      301 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      361 cgatgccacc tacggcaagc tgaccctgaa gctgatctgc accaccggca agctgcccgt
      421 gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg cccgctaccc
      481 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      541 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      601 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      661 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcaccgccga
      721 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg
      781 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      841 gcccgacaac cactacctga gctaccagtc caagctgagc aaagacccca acgagaagcg
      901 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg ggtaccaagg tgtacgaccc
      961 cgagcagagg aagaggatga tcaccggccc ccagtggtgg gccaggtgca agcagatgaa
     1021 cgtgctggac agcttcatca actactacga cagcgagaag cacgccgaga acgccgtgat
     1081 cttcctgcac ggcaacgcca ctagcagcta cctgtggagg cacgtggtgc cccacatcga
     1141 gcccgtggcc aggtgcatca tccccgatct gatcggcatg ggcaagagcg gcaagagcgg
     1201 caacggcagc tacaggctgc tggaccacta caagtacctg accgcctggt tcgagcttct
     1261 gaacctgccc aagaagatca tcttcgtggg ccacgactgg ggcgccgccc tggccttcca
     1321 ctacgcctac gagcaccagg acaggatcaa ggccatcgtg cacatggaga gcgtggtgga
     1381 cgtgatcgag agctgggacg agtggccaga catcgaggag gacatcgccc tgatcaagag
     1441 cgaggagggc gagaagatgg tgctggagaa caacttcttc gtggagaccg tgctgcccag
     1501 caagatcatg agaaagctgg agcccgagga gttcgccgcc tacctggagc ccttcaagga
     1561 gaagggcgag gtgagaagac ccaccctgag ctggcccaga gagatccccc tggtgaaggg
     1621 cggcaagccc gacgtggtgc agatcgtgag aaactacaac gcctacctga gagccagcga
     1681 cgacctgccc aagctgttca tcgagggcga ccccggcttc ttcagcaacg ccatcgtgga
     1741 gggcgccaag aagttcccca acaccgagtt cgtgaaggtg aagggcctgc acttcctcca
     1801 ggaggacgcc cccgacgaga tgggcaagta catcaagagc ttcgtggaga gagtgctgaa
     1861 gaacgagcag taagaattcg aagcttgatc cggctgctaa caaagcccga aaggaagctg
     1921 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     1981 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atctggcgta atagcgaaga
     2041 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     2101 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     2161 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     2221 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     2281 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     2341 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     2401 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     2461 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     2521 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     2581 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2641 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     2701 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     2761 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     2821 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     2881 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     2941 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3001 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3061 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3121 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3181 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3241 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3301 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3361 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     3421 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     3481 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     3541 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     3601 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3661 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3841 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
