LOCUS       sequence-139184-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 3A2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-3A2-mCherry
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..47)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagg
       61 gtaccttatt tgtacagttc atccataccg ccggtagagt ggcggccttc tgcacgttcg
      121 tactgttcaa cgatcgtata gtcttcgtta tggctggtga tatccagctt gatgttcacg
      181 ttgtatgcac ccggcagttg gaccggcttt ttagccttgt acgtggtttt cacttctgcg
      241 tcatagtgac cgccatcctt cagtttcagg cgctgcttga tttcgccttt cagggcaccg
      301 tcttccgggt acatacgttc ggaactcgct tcccagccca tcgtcttttt ctgcataacc
      361 ggaccatcgc tcgggaaatt ggtgccgcgc agcttcactt tataaataaa ttcaccgtct
      421 tgcagagagc tatcctgcgt gacggtaacc acaccgccat cttcgaagtt cataacacgt
      481 tcccatttga agccttccgg aaaggacagt ttcagatagt ccgggatatc ggccggatgc
      541 ttaacgtacg ctttcgaacc atacatgaat tgcggtgaca gaatatccca agcaaacggc
      601 agcggaccgc ctttggtgac cttcagtttt gccgtctggg tgccttcata cggacggcct
      661 tcaccttcgc cttcaatttc aaattcgtga ccattgacgc tgccttccat gtggaccttg
      721 aaacgcataa attccttgat aatcgccatg ttgtcttctt cgcctt
//
