LOCUS       sequence-139185-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 1E4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-1E4-mCherry
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..46)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 acgagtccat gtgctggcgt tcaaatttcg cagcagcggt ttctttacca gactcgaggg
       61 taccttattt gtacagttca tccataccgc cggtagagtg gcggccttct gcacgttcgt
      121 actgttcaac gatcgtatag tcttcgttat ggctggtgat atccagcttg atgttcacgt
      181 tgtatgcacc cggcagttgg accggctttt tagccttgta cgtggttttc acttctgcgt
      241 catagtgacc gccatccttc agtttcaggc gctgcttgat ttcgcctttc agggcaccgt
      301 cttccgggta catacgttcg gaactcgctt cccagcccat cgtctttttc tgcataaccg
      361 gaccatcgct cgggaaattg gtgccgcgca gcttcacttt ataaataaat tcaccgtctt
      421 gcagagagct atcctgcgtg acggtaacca caccgccatc ttcgaagttc ataacacgtt
      481 cccatttgaa gccttccgga aaggacagtt tcagatagtc cgggatatcg gccggatgct
      541 taacgtacgc tttcgaacca tacatgaatt gcggtgacag aatatcccaa gcaaacggca
      601 gcggaccgcc tttggtgacc ttcagttttg ccgtctgggt gccttcatac ggacggcctt
      661 caccttcgcc ttcaatttca aattcgtgac cattgacgct gccttccatg tggaccttga
      721 aacgcataaa ttccttgata atcgccatgt tgtcttcttc gcctt
//
