LOCUS       sequence-139187-        7494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Myc tagged human CIN85 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Kawata A, Iida J, Ikeda M, Sato Y, Mori H, Kansaku A, Sumita K, 
            Fujiwara N, Rokukawa C, Hamano M, Hirabayashi S, Hata Y
  TITLE     CIN85 is localized at synapses and forms a complex with S-SCAM via 
            dendrin.
  JOURNAL   J Biochem. 2006 May;139(5):931-9.
  PUBMED    16751601
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Biochem."; date: "2006-05"; volume: "139(5)"; pages: "931-9"
COMMENT     Sequence Label: pClneoMyc human CIN85
FEATURES             Location/Qualifiers
     source          1..7494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1106..1135
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3198..3319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3209..3228)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3263..3282
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3505..3960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3592..3611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3802..3823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4077..4434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4077..4268
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4100..4120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4285..4420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4485..5279
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4539..4558)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5149..5168
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5343..5391
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5432..5451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5551..5573
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5611..5629)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5697..5801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5802..5870,5871..6662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6020..6039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6833..7421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7322..7341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc agacccccca acatggagca gaagcttatc agcgaggagg acctgctcga
     1141 ggaattcgtg gaggccatag tggagtttga ctaccaggcc cagcacgatg atgagctgac
     1201 gatcagcgtg ggtgaaatca tcaccaacat caggaaggag gatggaggct ggtgggaggg
     1261 acagatcaac ggcaggagag gtttgttccc tgacaacttt gtaagagaaa taaagaaaga
     1321 gatgaagaaa gaccctctca ccaacaaagc tccagaaaag cccctgcacg aagtgcccag
     1381 tggaaactct ttgctgtctt ctgaaacgat tttaagaacc aataagagag gcgagcgacg
     1441 gaggcgccgg tgccaggtgg cattcagcta cctgccccag aatggcgatg aacttgagct
     1501 gaaagttggc gacatcatag aggtggtagg agaggtagag gaaggatggt gggaaggtgt
     1561 tctcaacggg aagactggaa tgtttccttc caacttcatc aaggagctgt caggggagtc
     1621 ggatgagctt ggcatttccc aggatgagca gctatccaag tcaagtttaa gggaaaccac
     1681 aggctccgag agtgatgggg gtgactcaag cagcaccaag tctgaaggtg ccaacgggac
     1741 agtggcaact gcagcaatcc agcccaagaa agttaaggga gtgggctttg gagacatttt
     1801 caaagacaag ccaatcaaac taagaccaag gtcaattgaa gtagaaaatg actttctgcc
     1861 ggtagaaaag actattggga agaagttacc tgcaactaca gcaactccag actcatcaaa
     1921 aacagaaatg gacagcagga caaagagcaa ggattactgc aaagtaatat ttccatatga
     1981 gtcacagaat gatgatgaat tgacaatcaa agaaggagat atagtcactc tcatcaataa
     2041 ggactgcatc gacgtaggct ggtgggaagg agagctgaac ggcagacgag gcgtgttccc
     2101 cgataacttc gtgaagttac ttccaccgga ctttgaaaag gaagggaata gacccaagaa
     2161 gccaccgcct ccatccgctc ctgtcatcaa acaaggggca ggcaccactg agagaaaaca
     2221 tgaaattaaa aagatacctc ctgaaagacc agaaatgctt ccaaacagaa cagaagaaaa
     2281 agaaagacca gagagagagc caaaactgga tttacagaag ccctccgttc ctgccatacc
     2341 gccaaaaaag cctcggccac ctaagaccaa ttctctcagc agacctggcg cactgccccc
     2401 gagaaggccg gagagaccgg tgggtccgct gacacacacc aggggtgaca gtccaaagat
     2461 tgacttggcc ggcagttcgc tatctggcat cctggacaaa gatctctcgg accgcagcaa
     2521 tgacattgac ttagaaggtt ttgactccgt ggtatcatct actgagaaac tcagtcatcc
     2581 gaccacaagc agaccaaaag ctacagggag gcggcctccg tcccagtccc tcacatcttc
     2641 atccctttca agccctgata tcttcgactc cccaagtccc gaagaggata aggaggaaca
     2701 catttcactt gcgcacagag gagtggacgc gtcaaagaaa acttccaaga ctgttaccat
     2761 atcccaagtg tctgacaaca aagcatccct gccgcccaag ccggggacca tggcagcagg
     2821 tggcggtggg ccagcccctc tgtcctcagc ggcgccctcc cccctgtcat cctctttggg
     2881 aacagctgga cacagagcca actccccgtc tctgttcggc acggaaggaa aaccaaagat
     2941 ggagcctgcg gccagcagcc aggcggccgt ggaggagcta aggacacagg tccgcgagct
     3001 gaggagcatc atcgagacca tgaaggacca gcagaaacga gagattaaac agttattgtc
     3061 tgagttggat gaagagaaga aaatccggct tcggttgcag atggaagtga acgacataaa
     3121 gaaagctcta caatcaaaat agtcgacccg ggcggccgct tccctttagt gagggttaat
     3181 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3301 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3361 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatccgata
     3421 aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3481 ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3661 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3901 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgcggatc
     4021 tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     4081 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     4381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     4441 gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt gaacaagatg
     4501 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4561 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4621 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     4681 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4741 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4801 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4861 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4921 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4981 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     5041 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5101 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5161 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5221 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     5281 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgatggcc
     5341 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     5401 cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5461 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5521 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5581 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5641 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6481 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6661 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6721 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6781 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6841 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6901 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6961 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7021 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7081 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7141 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7201 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7261 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7321 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7381 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7441 ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag atct
//
