LOCUS       sequence-139190-         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Violacein pathway (vioABECD) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux 
            redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC-3A2-vioD
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaccgaaata cccaaaacac attggatacg acaacaaata ccgtgtttca attcggccag
       61 ccggttgaca ttgacttgcc gccggcacgt ttccacgaat acctccactc acttggtcca
      121 aaagttaatc gctttggtaa aaaagatgaa aatggagaat tcagtgtagc gtgtggctac
      181 aacatgcttg gtaataatca tttttcatgg cacgacgcta aagttacggc tttgcaattg
      241 cgcccgggtg attgtgttgt tcaagatcca ctcatagggt gttctgttaa tttatgggga
      301 cactacaatg agtaccttag aacctcattc aatcgtgctc gctgggttga taatgacccg
      361 acccgaagtg actcggcact catctatgct ggacaaatta cattaactgc aaatggcagt
      421 gaggcaagtg aaaagcatgt gatgtctgct gacattgatt gtacacatgg agtgagatgg
      481 ataaatccta gctttattaa tagcctcgac gaacatgcgt ttgctgaaga tattgcagca
      541 gcccgtttgt ttcagtttgc tgtcaataag aacagcaagg atttcttatt gaatcaattg
      601 ggcttgcagt cagaatttat tgaacaatta cgaatagcac tagaacaaga agatgttaag
      661 ggcttagttg ttcaatattg tgtaagcaat ctatcgcctc ctgtagcgcc caatatgccg
      721 gtctt
//
