LOCUS       sequence-139191-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 5F5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-5F5
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(290..368)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
       61 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
      121 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga
      181 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc
      241 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg
      301 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga
      361 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
      421 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
      481 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
      541 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaa
//
