LOCUS       sequence-139193-         491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 1D4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-1D4
FEATURES             Location/Qualifiers
     source          1..491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(246..324)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
       61 cgggcctctt gcgggattac gaaatcatcc tgtggagctt agtaggttta gcaagatggc
      121 agcgcctaaa tgtagaatga taaaaggatt aagagattaa tttccctaaa aatgataaaa
      181 caagcgtttt gaaagcgctt gtttttttgg tttgcagtca gagtagaata gaagtatcaa
      241 aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct tattttaact
      301 tgctatgctg ttttgaatgg ttccaacaag attattttat aacttttata acaaataatc
      361 aaggagaaat tcaaagaaat ttatcagcca taaaacaata cttaatacta tagaatgata
      421 acaaaataaa ctacttttta aaagaatttt gtgttataat ctatttatta ttaagtattg
      481 ggtaatattt t
//
