LOCUS       sequence-139195-        7985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Dimitrova N, Chen YC, Spector DL, de Lange T
  TITLE     53BP1 promotes non-homologous end joining of telomeres by increasing
            chromatin mobility.
  JOURNAL   Nature. 2008 Nov 27;456(7221):524-8. doi: 10.1038/nature07433. Epub 
            2008 Oct 19.
  PUBMED    18931659
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07433"; journalName: "Nature"; date: "2008-11-27- 
            27"; volume: "456"; issue: "7221"; pages: "524-8"
COMMENT     Sequence Label: mCherry-BP1-2 pLPC-Puro
FEATURES             Location/Qualifiers
     source          1..7985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(982..1773,1774..1842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1605..1624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1843..1947)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1978..2447
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2512..2711
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2912..3328
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3258..3280
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3296..3312
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3346..3945
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3346..3365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3842..3862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4034..4053)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4225..4604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4605..4808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4759..4779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4853..4872
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4853..4871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4905..4929
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4950..4959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4956..5663
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5101..5119)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5369..5388
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(7171..7195)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             7449..7918
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgc aaatcaatct aaagtatata
      961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1261 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcagcctg
     1981 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     2041 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     2101 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     2161 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     2221 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     2281 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     2341 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     2401 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     2461 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg caagctggcc
     2521 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
     2581 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
     2641 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
     2701 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
     2761 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
     2821 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     2881 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     2941 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     3001 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     3061 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     3121 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     3181 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     3241 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     3301 tccagccctc actccttctc taggcgccgg ccggatcagc ttaccatgac cgagtacaag
     3361 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3421 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3481 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3541 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3601 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3661 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3721 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3781 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3841 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3901 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     3961 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cggatctccc gatcccctat
     4021 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     4081 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     4141 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     4201 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     4261 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     4321 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     4381 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     4441 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     4501 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     4561 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     4621 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     4681 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     4741 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     4801 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
     4861 ctcactatag ggagacccaa gcttggtacc gagctcggat catcacaagt ttgtacaaaa
     4921 aagcaggctc cgcggccgcc cccttcaccg ccaccatggt gagcaagggc gaggaggata
     4981 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     5041 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5101 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     5161 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     5221 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5281 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5341 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5401 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5461 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     5521 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     5581 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     5641 gcggcatgga cgagctgtac aagggatccc agggagaaga agagtttgat atgcctcagc
     5701 ctccacatgg ccatgtctta catcgtcaca tgagaacaat ccgggaagta cgcacacttg
     5761 tcactcgtgt cattacagat gtgtattatg tggatggaac agaagtagaa agaaaagtaa
     5821 ctgaggagac tgaagagcca attgtagagt gtcaggagtg tgaaactgaa gtttcccctt
     5881 cacagactgg gggctcctca ggtgacctgg gggatatcag ctccttctcc tccaaggcat
     5941 ccagcttaca ccgcacatca agtgggacaa gtctctcagc tatgcacagc agtggaagct
     6001 cagggaaagg agccggacca ctcagaggga aaaccagcgg gacagaaccc gcagattttg
     6061 ccttacccag ctcccgagga ggcccaggaa aactgagtcc tagaaaaggg gtcagtcaga
     6121 cagggacgcc agtgtgtgag gaggatggtg atgcaggcct tggcatcaga cagggaggga
     6181 aggctccagt cacgcctcgt gggcgtgggc gaaggggccg cccaccttct cggaccactg
     6241 gaaccagaga aacagctgtg cctggcccct tgggcataga ggacatttca cctaacttgt
     6301 caccagatga taaatccttc agccgtgtcg tgccccgagt gccagactcc accagacgaa
     6361 cagatgtggg tgctggtgct ttgcgtcgta gtgactctcc agaaattcct ttccaggctg
     6421 ctgctggccc ttctgatggc ttagatgcct cctctccagg aaatagcttt gtagggctcc
     6481 gtgttgtagc caagtggtca tccaatggct acttttactc tgggaaaatc acacgagatg
     6541 tcggagctgg gaagtataaa ttgctctttg atgatgggta cgaatgtgat gtgttgggca
     6601 aagacattct gttatgtgac cccatcccgc tggacactga agtgacggcc ctctcggagg
     6661 atgagtattt cagtgcagga gtggtgaaag gacataggaa ggagtctggg gaactgtact
     6721 acagcattga aaaagaaggc caaagaaagt ggtataagcg aatggctgtc atcctgtcct
     6781 tggagcaagg aaacagactg agagagcagt atgggcttgg cccctatgaa gcagtaacac
     6841 ctcttacaaa ggcagcagat atcagcttag acaatttggt ggaagggaag cggaaacggc
     6901 gcagtaacgt cagctcccca gccaccccta ctgcctccag tagcagcagc acaaccccta
     6961 cccgaaagat cacagaaagt cctcgtgcct ccatgggagt tctctcaggc aaaagaaaac
     7021 ttatcacttc tgaagaggaa cggtcccctg ccaagcgagg tcgcaagtct gccacagtaa
     7081 aacctggtgc agtaggggca ggagagtttg tgagcccctg tgagagtgga gacaacaccg
     7141 gtgaatgagg atccaagggt gggcgcgccg acccagcttt cttgtacaaa gtggtgatga
     7201 tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc
     7261 gctcgagcat gcatctagag ggccgataaa ataaaagatt ttatttagtc tccagaaaaa
     7321 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
     7381 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     7441 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     7501 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     7561 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
     7621 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
     7681 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
     7741 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
     7801 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
     7861 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
     7921 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
     7981 agttg
//
